Molecular engineering to improve lignocellulosic biomass based applications using filamentous fungi.

2区生物学 Q1 Immunology and Microbiology

Advances in applied microbiology Pub Date : 2021-01-01 Epub Date: 2020-10-17 DOI:10.1016/bs.aambs.2020.09.001

Jiali Meng, Miia R Mäkelä, Ronald P de Vries

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引用次数: 8

Abstract

Lignocellulosic biomass is an abundant and renewable resource, and its utilization has become the focus of research and biotechnology applications as a very promising raw material for the production of value-added compounds. Filamentous fungi play an important role in the production of various lignocellulolytic enzymes, while some of them have also been used for the production of important metabolites. However, wild type strains have limited efficiency in enzyme production or metabolic conversion, and therefore many efforts have been made to engineer improved strains. Examples of this are the manipulation of transcriptional regulators and/or promoters of enzyme-encoding genes to increase gene expression, and protein engineering to improve the biochemical characteristics of specific enzymes. This review provides and overview of the applications of filamentous fungi in lignocellulosic biomass based processes and the development and current status of various molecular engineering strategies to improve these processes.

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利用丝状真菌改进木质纤维素生物质应用的分子工程。

木质纤维素生物质是一种丰富的可再生资源，其利用已成为生物技术研究和应用的热点，是生产高附加值化合物的极具前景的原料。丝状真菌在生产各种木质纤维素水解酶方面发挥着重要作用，其中一些也被用于生产重要的代谢物。然而，野生型菌株在产酶或代谢转化方面的效率有限，因此人们已经做出了许多努力来改造菌株。这方面的例子是操纵转录调控因子和/或酶编码基因的启动子来增加基因表达，以及蛋白质工程来改善特定酶的生化特性。本文综述了丝状真菌在木质纤维素生物质过程中的应用，以及改善这些过程的各种分子工程策略的发展和现状。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Advances in applied microbiology 生物-生物工程与应用微生物

CiteScore

8.20

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Advances in Applied Microbiology offers intensive reviews of the latest techniques and discoveries in this rapidly moving field. The editors are recognized experts and the format is comprehensive and instructive. Published since 1959, Advances in Applied Microbiology continues to be one of the most widely read and authoritative review sources in microbiology. Recent areas covered include bacterial diversity in the human gut, protozoan grazing of freshwater biofilms, metals in yeast fermentation processes and the interpretation of host-pathogen dialogue through microarrays.