Evaluation of the Acute Hepatoprotective Potential of Hydroethanolic Extract of Duranta erecta L. Parts.

IF 3.4 Q2 TOXICOLOGY
Journal of Toxicology Pub Date : 2020-12-09 eCollection Date: 2020-01-01 DOI:10.1155/2020/8815719
Shadrack Donkor, Christopher Larbie, Gustav Komlaga, Benjamin Obukowho Emikpe
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引用次数: 8

Abstract

Liver disease is a major health problem and its treatment is costly in most developing countries with attendant adverse effects. This study aimed at determining the acute hepatoprotective efficacy of Duranta erecta hydroethanolic extracts of leaves, ripe and unripe fruits against CCl4-, and acetaminophen-induced hepatotoxicity in animals. Materials and Methods. CCl4 (1 mL/kg body weight in olive oil) and acetaminophen (500 mg/kg b.wt) were used to induce hepatotoxicity in the animals. Animals were treated with extracts at 250 mg/kg b.wt and standard drug, silymarin (100 mg/kg), for 7 days. Hepatoprotective efficacy was assessed by assaying serum biochemical markers such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (γGT), bilirubin (Bil), antioxidative biomarkers including reduced glutathione (GSH), glutathione peroxidase (GPx), glutathione transferase (GST), superoxide dismutase (SOD), malondialdehyde (MDA), hydrogen peroxidase (H202), and nitric oxide (NO), as well as histological observations. Results. Exposure of the animals to CCl4 and acetaminophen resulted in liver injury as evidenced by elevated ALT, AST, ALP, γGT, Bil, MDA, H2O2, and NO levels with resultant derangement in liver microarchitecture. Pretreatment with hydroethanolic extracts, particularly ripe fruits of Duranta erecta, led to a reduction in these indicators and an increase in GSH, GPx, GST, and SOD levels. Biochemical data were supported by improvement in liver structure. Conclusion. The findings suggest that hydroethanolic Duranta erecta ripe fruits extract possesses hepatoprotective and antioxidative activities against CCl4- and acetaminophen-induced toxicity and could be developed as a potent agent for drug-induced liver diseases.

杜鹃花部分水乙醇提取物急性肝保护作用的评价。
肝病是一个主要的健康问题,在大多数发展中国家,其治疗费用昂贵,并伴有副作用。本研究旨在探讨直立杜鹃叶片、成熟果实和未成熟果实氢乙醇提取物对CCl4-和对乙酰氨基酚引起的动物急性肝毒性的保护作用。材料与方法。用CCl4(橄榄油中1 mL/kg体重)和对乙酰氨基酚(500 mg/kg b.wt)诱导动物肝毒性。动物分别用250 mg/kg b.wt的提取物和标准药物水飞蓟素(100 mg/kg)治疗7天。通过测定血清生化指标如丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、碱性磷酸酶(ALP)、γ -谷氨酰转移酶(γ - gt)、胆红素(Bil)、抗氧化生物标志物如还原性谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GPx)、谷胱甘肽转移酶(GST)、超氧化物歧化酶(SOD)、丙二醛(MDA)、过氧化氢酶(H202)和一氧化氮(NO),以及组织学观察来评估肝保护作用。结果。小鼠暴露于CCl4和对乙酰氨基酚后,ALT、AST、ALP、γ - gt、Bil、MDA、H2O2和NO水平升高,导致肝脏微结构紊乱,从而导致肝损伤。预处理水乙醇提取物,特别是成熟的杜鹃果实,导致这些指标的降低和GSH, GPx, GST和SOD水平的增加。肝脏结构的改善支持了生化数据。结论。结果表明,水乙醇提取物对CCl4和对乙酰氨基酚具有抗肝保护和抗氧化作用,可作为药物性肝病的有效药物。
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来源期刊
Journal of Toxicology
Journal of Toxicology TOXICOLOGY-
CiteScore
5.50
自引率
3.40%
发文量
0
审稿时长
10 weeks
期刊介绍: Journal of Toxicology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of toxicological sciences. The journal will consider articles looking at the structure, function, and mechanism of agents that are toxic to humans and/or animals, as well as toxicological medicine, risk assessment, safety evaluation, and environmental health.
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