Effect of dietary inclusion of dried apple pomace on faecal butyrate concentration and modulation of gut microbiota in dogs.

Abstract

This research aimed to evaluate the apparent total tract digestibility (ATTD) of nutrients, metabolisable energy (ME) and palatability of the diet, as well as products of intestinal fermentation and faecal microbiota of dogs fed with dried apple. For this purpose, three experiments were performed. In Experiment I, digestibility and ME of four diets containing 0%, 3%, 6% and 9% dried apple were evaluated, in addition to the faecal characteristics of the dogs. The diets were offered to eight adult dogs, distributed in double Latin square (4 × 4), totalling eight repetitions per treatment. In Experiment II, products of intestinal fermentation and faecal microbiota from 16 adult dogs fed diets containing 0% and 9% dried apple for 30 d (n = 8) were evaluated. Finally, Experiment III compared the dietary preference of 0 vs. 9% dried apple using 15 adult dogs. The inclusion of dried apple in the diet (p < 0.05) showed a linear reduction in the ATTD of dry matter (DM), crude protein (CP), and acid hydrolysed ether extract (EEA), and a linear increase in the ATTD of total dietary fibre (TDF). Consumption of 9% of dried apple increased faecal butyrate and reduced propionate and ammonia (p < 0.05). With this diet, there was also an increase (p < 0.05) in the faecal concentration of Faecalibacterium, Erysipelatoclostridium, Blautia, and Bacteroides. No differences were found in the palatability of the diets. The inclusion of up to 9% of dried apple in the diet reduces the digestibility of nutrients and does not influence the dogs' food preference; however, it improves some indicators of dogs' intestinal functionality.