Neferine improves oxidative stress and apoptosis in benign prostate hyperplasia via Nrf2-ARE pathway.

IF 5.2 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Nabila Jahan, Apu Chowdhury, Ting Li, Ke Xu, Fen Wei, Sicen Wang
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引用次数: 16

Abstract

Background: Progression of Benign Prostate hyperplasia (BPH) is vulnerable to oxidative stress (OS) and prostatic enlargement among the aging males through apoptosis deregulation. Our present study aimed to investigate the effect of neferine (NF) in the regulation of oxidative stress and apoptosis in human BPH-1 cells.

Methods: BPH epithelial cell line BPH-1 was treated with NF for 24 and 48 h. To measure oxidative stress (OS) we investigated MDA, SOD, and GST expression along with Nrf2 and its downstream gene and protein expression. Cell proliferation and apoptosis regulation was assayed with respective methods.

Results: Investigation revealed NF remarkably activate Nrf2 and its downstream proteins HO-1 and NQO1 at 48 h more substantially. Nrf2/Keap1 relative gene and protein expression indicated that NF might trigger Nrf2 upregulation by decreasing Keap1 expression. Both NF concentrations (3 µM and 9 µM) were able to deplete ROS and lipid peroxidation, concurrently, up-regulated SOD and GST. NF reduced cell proliferation significantly along with the regulation of apoptotic proteins Bax, Bcl2, Cyt-C, Caspase 9, and Caspase 3 at the same time (48 h).

Conclusion: This study is the first to manifest that NF may potentially regulate BPH by counterbalancing between OS and apoptosis through the activation of Nrf2-ARE pathway.

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莲子碱通过Nrf2-ARE途径改善良性前列腺增生的氧化应激和细胞凋亡。
背景:男性良性前列腺增生(BPH)在衰老过程中易受氧化应激(OS)和前列腺增大的影响。本研究旨在探讨neferine (NF)在调节人BPH-1细胞氧化应激和凋亡中的作用。方法:用NF处理BPH上皮细胞株BPH-1 24和48 h,测定氧化应激(OS)水平,检测MDA、SOD、GST以及Nrf2及其下游基因和蛋白的表达。用不同的方法检测细胞增殖和凋亡调节。结果:研究发现,NF在48 h显著激活Nrf2及其下游蛋白HO-1和NQO1。Nrf2/Keap1相关基因及蛋白表达表明,NF可能通过降低Keap1的表达而触发Nrf2上调。两种NF浓度(3µM和9µM)均能减少ROS和脂质过氧化,同时上调SOD和GST。NF可显著降低细胞增殖,同时调节凋亡蛋白Bax、Bcl2、Cyt-C、Caspase 9和Caspase 3 (48 h)。结论:本研究首次证实NF可能通过激活Nrf2-ARE通路,在OS和凋亡之间进行平衡,从而调控BPH。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Redox Report
Redox Report 生物-生化与分子生物学
CiteScore
6.10
自引率
0.00%
发文量
28
审稿时长
>12 weeks
期刊介绍: Redox Report is a multidisciplinary peer-reviewed open access journal focusing on the role of free radicals, oxidative stress, activated oxygen, perioxidative and redox processes, primarily in the human environment and human pathology. Relevant papers on the animal and plant environment, biology and pathology will also be included. While emphasis is placed upon methodological and intellectual advances underpinned by new data, the journal offers scope for review, hypotheses, critiques and other forms of discussion.
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