Size-Dependent Bioactivity of Silver Nanoparticles: Antibacterial Properties, Influence on Copper Status in Mice, and Whole-Body Turnover.

IF 4.9 Q2 NANOSCIENCE & NANOTECHNOLOGY
Nanotechnology, Science and Applications Pub Date : 2020-12-31 eCollection Date: 2020-01-01 DOI:10.2147/NSA.S287658
Ekaterina A Skomorokhova, Tatiana P Sankova, Iurii A Orlov, Andrew N Savelev, Daria N Magazenkova, Mikhail G Pliss, Alexey N Skvortsov, Ilya M Sosnin, Demid A Kirilenko, Ivan V Grishchuk, Elena I Sakhenberg, Elena V Polishchuk, Pavel N Brunkov, Alexey E Romanov, Ludmila V Puchkova, Ekaterina Yu Ilyechova
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引用次数: 22

Abstract

Purpose: The ability of silver nanoparticles (AgNPs) of different sizes to influence copper metabolism in mice is assessed.

Materials and methods: AgNPs with diameters of 10, 20, and 75 nm were fabricated through a chemical reduction of silver nitrate and characterized by UV/Vis spectrometry, transmission and scanning electronic microscopy, and laser diffractometry. To test their bioactivity, Escherichia coli cells, cultured A549 cells, and C57Bl/6 mice were used. The antibacterial activity of AgNPs was determined by inhibition of colony-forming ability, and cytotoxicity was tested using the MTT test (viability, %). Ceruloplasmin (Cp, the major mammalian extracellular copper-containing protein) concentration and enzymatic activity were measured using gel-assay analyses and WB, respectively. In vitro binding of AgNPs with serum proteins was monitored with UV/Vis spectroscopy. Metal concentrations were measured using atomic absorption spectrometry.

Results: The smallest AgNPs displayed the largest dose- and time-dependent antibacterial activity. All nanoparticles inhibited the metabolic activity of A549 cells in accordance with dose and time, but no correlation between cytotoxicity and nanoparticle size was found. Nanosilver was not uniformly distributed through the body of mice intraperitoneally treated with low AgNP concentrations. It was predominantly accumulated in liver. There, nanosilver was included in ceruloplasmin, and Ag-ceruloplasmin with low oxidase activity level was formed. Larger nanoparticles more effectively interfered with the copper metabolism of mice. Large AgNPs quickly induced a drop of blood serum oxidase activity to practically zero, but after cancellation of AgNP treatment, the activity was rapidly restored. A major fraction of the nanosilver was excreted in the bile with Cp. Nanosilver was bound by alpha-2-macroglobulin in vitro and in vivo, but silver did not substitute for the copper atoms of Cp in vitro.

Conclusion: The data showed that even at low concentrations, AgNPs influence murine copper metabolism in size-dependent manner. This property negatively correlated with the antibacterial activity of AgNPs.

Abstract Image

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银纳米颗粒的大小依赖性生物活性:抗菌特性,对小鼠体内铜状态的影响,以及全身循环。
目的:研究不同粒径银纳米颗粒对小鼠铜代谢的影响。材料与方法:采用硝酸银化学还原法制备直径分别为10、20和75 nm的AgNPs,并采用紫外/可见光谱法、透射电镜和扫描电镜以及激光衍射法对其进行了表征。以大肠杆菌细胞、培养的A549细胞和C57Bl/6小鼠为实验对象,检测其生物活性。通过抑制菌落形成能力来检测AgNPs的抑菌活性,并采用MTT试验(活力,%)检测细胞毒性。用凝胶法测定铜蓝蛋白(Ceruloplasmin,哺乳动物主要的细胞外含铜蛋白)浓度,用WB法测定酶活性。用紫外/可见光谱法监测AgNPs与血清蛋白的体外结合。用原子吸收光谱法测定金属浓度。结果:最小的AgNPs表现出最大的剂量和时间依赖性抗菌活性。各纳米颗粒对A549细胞代谢活性的抑制作用均与剂量和时间有关,但细胞毒性与纳米颗粒大小无相关性。低AgNP腹腔注射小鼠体内纳米银分布不均匀。主要积聚在肝脏。在铜蓝蛋白中加入纳米银,形成低氧化酶活性的ag -铜蓝蛋白。更大的纳米颗粒更有效地干扰了小鼠的铜代谢。大剂量AgNP可迅速诱导血清氧化酶活性降至几乎为零,但取消AgNP处理后,活性迅速恢复。纳米银在体外和体内均与α -2巨球蛋白结合,但在体外不能替代Cp的铜原子。结论:即使在低浓度下,AgNPs也以大小依赖的方式影响小鼠的铜代谢。该特性与AgNPs的抑菌活性呈负相关。
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来源期刊
Nanotechnology, Science and Applications
Nanotechnology, Science and Applications NANOSCIENCE & NANOTECHNOLOGY-
CiteScore
11.70
自引率
0.00%
发文量
3
审稿时长
16 weeks
期刊介绍: Nanotechnology, Science and Applications is an international, peer-reviewed, Open Access journal that focuses on the science of nanotechnology in a wide range of industrial and academic applications. The journal is characterized by the rapid reporting of reviews, original research, and application studies across all sectors, including engineering, optics, bio-medicine, cosmetics, textiles, resource sustainability and science. Applied research into nano-materials, particles, nano-structures and fabrication, diagnostics and analytics, drug delivery and toxicology constitute the primary direction of the journal.
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