Study on the Dynamic Proliferation of JEV in BHK-21 Cells.

IF 3.2 4区医学 Q3 VIROLOGY

Intervirology Pub Date : 2021-01-05 DOI:10.1159/000510585

Fuliang Zhang, Jun Luo, Man Teng, Guangxu Xing, Junqing Guo, Yihua Zhang

{"title":"Study on the Dynamic Proliferation of JEV in BHK-21 Cells.","authors":"Fuliang Zhang, Jun Luo, Man Teng, Guangxu Xing, Junqing Guo, Yihua Zhang","doi":"10.1159/000510585","DOIUrl":null,"url":null,"abstract":"Introduction: Epidemic Japanese encephalitis is one of the most important zoonotic diseases that cause central nervous system damage. The vaccination has become the most effective and economical measure for its control. Hence, real-time monitoring of Japanese encephalitis virus (JEV) proliferation is crucial to optimize virus inoculation, culturing conditions, and virus harvest time.Methods: The proliferation dynamics of JEV in BHK-21 cells was studied by combining the established quantitative PCR method with the conventional TCID50 assay in this study.Results: The proliferation curve determined by the 2 methods has a definite parallel relationship, but the quantitative real-time PCR method (4 h) is faster and more sensitive than the TCID50 method (3-4 days). The determination results of TCID50 showed that the highest viral titer was 105.44 TCID50/0.1 mL and 104.86 TCID50/0.1 mL in cell suspension and culture supernate, respectively, while the virus RNA copies reached the peak at 1.0 × 107.5 copies/µL and 1.0 × 105.6 copies/µL in cell suspension and culture supernate, respectively.Conclusion: The comprehensive analysis showed that the best time for JEV proliferation in BHK-21 cell was 60 h post infection.","PeriodicalId":14547,"journal":{"name":"Intervirology","volume":null,"pages":null},"PeriodicalIF":3.2000,"publicationDate":"2021-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Intervirology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1159/000510585","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VIROLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction: Epidemic Japanese encephalitis is one of the most important zoonotic diseases that cause central nervous system damage. The vaccination has become the most effective and economical measure for its control. Hence, real-time monitoring of Japanese encephalitis virus (JEV) proliferation is crucial to optimize virus inoculation, culturing conditions, and virus harvest time.

Methods: The proliferation dynamics of JEV in BHK-21 cells was studied by combining the established quantitative PCR method with the conventional TCID50 assay in this study.

Results: The proliferation curve determined by the 2 methods has a definite parallel relationship, but the quantitative real-time PCR method (4 h) is faster and more sensitive than the TCID50 method (3-4 days). The determination results of TCID50 showed that the highest viral titer was 105.44 TCID50/0.1 mL and 104.86 TCID50/0.1 mL in cell suspension and culture supernate, respectively, while the virus RNA copies reached the peak at 1.0 × 107.5 copies/µL and 1.0 × 105.6 copies/µL in cell suspension and culture supernate, respectively.

Conclusion: The comprehensive analysis showed that the best time for JEV proliferation in BHK-21 cell was 60 h post infection.

查看原文本刊更多论文

研究 JEV 在 BHK-21 细胞中的动态增殖。

导言：流行性日本脑炎是导致中枢神经系统损伤的最重要的人畜共患病之一。接种疫苗已成为控制该病最有效、最经济的措施。因此，实时监测日本脑炎病毒（JEV）的增殖对优化病毒接种、培养条件和病毒收获时间至关重要：方法：本研究结合已建立的定量 PCR 方法和传统的 TCID50 检测方法，研究了 JEV 在 BHK-21 细胞中的增殖动态：结果：两种方法测定的增殖曲线具有明确的平行关系，但实时定量 PCR 法（4 h）比 TCID50 法（3-4 天）更快、更灵敏。TCID50 的测定结果显示，细胞悬液和培养上清液中的病毒滴度最高分别为 105.44 TCID50/0.1 mL 和 104.86 TCID50/0.1 mL，而病毒 RNA 拷贝的峰值分别为 1.0 × 107.5 拷贝/μL 和 1.0 × 105.6 拷贝/μL：综合分析表明，JEV 在 BHK-21 细胞中增殖的最佳时间是感染后 60 h。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Intervirology 医学-病毒学

CiteScore

5.40

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： ''Intervirology'' covers progress in both basic and clinical virus research, and aims to provide a forum for the various disciplines within virology. Issues publishing original papers alternate with thematic issues, focusing on clearly defined topics. This thematic concentration serves to make timely reviews, research reports and controversy easily accessible to both specialists in the field and those who want to keep track of the latest developments outside their own area of interest. In addition to original papers, regular issues publish short communications and letters to the editor to provide readers with a forum for the exchange of ideas and comments. The scope encompasses work on the molecular biology of human and animal viruses, including genome organization and regulation, and the structure and function of viral proteins. The pathogenesis, immunology, diagnosis, epidemiology, prophylaxis and therapy of viral diseases are considered.