The effect of T helper (Th)/T cytotoxic (Tc) ratio on disease severity in type A hemophilia patients.

Q3 Medicine

Human Antibodies Pub Date : 2021-01-01 DOI:10.3233/HAB-200434

Hossein Ali Khazaei, Majid Naderi, Ghasem Miri Aliabad, Seyed Mohammad Nasiraldin Tabatabaei, Ali Alidadi, Nezar Ali Moulaei, Mohammad Safdari, Hedayatollah Nahvi, Nader Cohan, Javid Dehghan, Ahmad Mehraban, Arman Jalili, Amin Khazaei, Ehsan Khazaei, Bahman Khazaei, Behnam Khazaei, Nima Rezaei, Morteza Salarzaie, Gholamreza Soleimani

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引用次数: 0

Abstract

Background and objectives: The aim of this study was to investigate the T helper (Th) to T cytotoxic (Tc) ratio in children suffering from type A hemophilia disease and to evaluate the correlation of this ratio with disease severity.

Material and method: Two mls of EDTA anti coagulated whole blood was collected. Immunophenotyping of lymphocytes count was carried out by FACS analysis using a double CD4 and CD8 kit. The mean ± SD of absolute numbers of CD4 and CD8 lymphocytes/ml was calculated and the ratio of CD4/CD8 was evaluated by statistical method.

Results: Among 80 type A hemophilia patients, 66 (82.5%) were male. The mean age was 15 ± 3.51 years. 12 (15%) of them were suffering from mild disease and 68 (85%) had sever disease. The CD4 /CD8 ratio was obtained between 0.45 and 1.44 with mean1.79 ± 0.78. The correlation between this ration and disease severity was 0.019.

Conclusion: The results showed that CD4/CD8 ratio has correlation with disease severity in type A hemophilia patients, however there was no association between this ratio and gender.

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辅助T (Th)/T细胞毒(Tc)比值对A型血友病患者病情严重程度的影响

背景与目的:本研究的目的是探讨儿童A型血友病患者T辅助(Th)与T细胞毒性(Tc)的比值，并评价该比值与疾病严重程度的相关性。材料与方法:采集EDTA抗凝全血2 ml。使用双CD4和CD8试剂盒，通过FACS分析淋巴细胞计数的免疫表型。计算CD4和CD8淋巴细胞绝对数目/ml的平均值±SD，并采用统计学方法评价CD4/CD8比值。结果:80例A型血友病患者中，男性66例，占82.5%。平均年龄15±3.51岁。其中轻症12例(15%)，重症68例(85%)。CD4 /CD8比值在0.45 ~ 1.44之间，平均值为1.79±0.78。该比例与疾病严重程度的相关系数为0.019。结论:A型血友病患者CD4/CD8比值与病情严重程度相关，但与性别无关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Human Antibodies Medicine-Immunology and Allergy

CiteScore

3.50

自引率

0.00%

发文量

期刊介绍： Human Antibodies is an international journal designed to bring together all aspects of human hybridomas and antibody technology under a single, cohesive theme. This includes fundamental research, applied science and clinical applications. Emphasis in the published articles is on antisera, monoclonal antibodies, fusion partners, EBV transformation, transfections, in vitro immunization, defined antigens, tissue reactivity, scale-up production, chimeric antibodies, autoimmunity, natural antibodies/immune response, anti-idiotypes, and hybridomas secreting interesting growth factors. Immunoregulatory molecules, including T cell hybridomas, will also be featured.