MiR-34c regulates the proliferation and apoptosis of lung cancer cells.

IF 1.2 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
Xianliang Jiang, Ming Li, Li Ke
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引用次数: 3

Abstract

Purpose: As miR-34c acts as a tumor suppressant for multiple cancers, the purpose of this study was to investigate that role that miR-34c plays in the proliferation and apoptosis of lung cancer.

Methods: The expression of miR-34c in 600 patients with lung cancer was quantitatively analyzed with real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) technology and correlated to clinical pathological parameters. The CCK-8 analysis and flow cytometry were carried out to detect cell proliferation and apoptosis in miR-34c-mimic transfected cell lines. Moreover, the regulation of miR-34c to interleukin-6 (IL-6) in cell lines was detected by western blot, qRT-PCR and dual-luciferase reporter assay.

Results: The expression of miR-34c was downregulated in lung cancer compared with adjacent normal tissues. The expression level of miR-34c was linked to stromal invasion. Furthermore, overexpressing miR-34c played an active role in effectively inhibiting cell proliferation and inducing apoptosis. In addition, a significant inverse relationship was exhibited between the expression of miR-34c and IL-6 in tumor tissues.

Conclusion: At the molecular level, IL-6 can be used as a direct target of miR-34c in the treatment of lung cancer cells and miR-34c can be used as an effective biomarker and therapeutic target for lung cancer.

MiR-34c调控肺癌细胞的增殖和凋亡。
目的:由于miR-34c在多种癌症中具有抑瘤作用,本研究的目的是探讨miR-34c在肺癌细胞增殖和凋亡中的作用。方法:采用实时定量逆转录聚合酶链反应(qRT-PCR)技术,定量分析600例肺癌患者中miR-34c的表达,并与临床病理参数进行相关性分析。通过CCK-8分析和流式细胞术检测转染miR-34c-mimic细胞系的细胞增殖和凋亡情况。此外,通过western blot、qRT-PCR和双荧光素酶报告基因法检测细胞中miR-34c对白细胞介素-6 (IL-6)的调控。结果:肺癌组织中miR-34c的表达较癌旁正常组织下调。miR-34c的表达水平与基质侵袭有关。此外,过表达miR-34c在有效抑制细胞增殖和诱导细胞凋亡方面发挥了积极作用。此外,肿瘤组织中miR-34c与IL-6的表达呈显著负相关。结论:在分子水平上,IL-6可作为miR-34c治疗肺癌细胞的直接靶点,miR-34c可作为肺癌有效的生物标志物和治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Clinical and Investigative Medicine
Clinical and Investigative Medicine 医学-医学:研究与实验
CiteScore
1.50
自引率
12.50%
发文量
18
审稿时长
>12 weeks
期刊介绍: Clinical and Investigative Medicine (CIM), publishes original work in the field of Clinical Investigation. Original work includes clinical or laboratory investigations and clinical reports. Reviews include information for Continuing Medical Education (CME), narrative review articles, systematic reviews, and meta-analyses.
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