Havachoobe (Onosma dichroanthum Boiss) Root Extract Decreases the Hepatitis B Virus Surface Antigen Secretion in the PLC/PRF/5 Cell Line.

IF 3.2 4区医学 Q3 VIROLOGY

Intervirology Pub Date : 2020-12-15 DOI:10.1159/000512140

Alireza Mohebbi, Fahimeh Azadi, Mohammad Mostakhdem Hashemi, Fatemeh Sana Askari, Nazanin Razzaghi

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引用次数: 0

Abstract

Background: Many efforts are currently focused on functional treatment of the hepatitis B virus (HBV). This can be done by suppressing the secretion of HBV surface antigen (HBsAg). Scientific communities are very interested in natural products in that respect.

Objective: Use of root extract of Havachoobe (Onosma dichroanthum BoissI), a Northern Iranian native medical herb, for assessment of its anti-HBsAg secretion activity.

Methods: Havachoobe had been bought at a nearby apothecary store. Plant root extract was obtained using a hydroalcoholic process. Cytotoxic activity of the extract was examined on PLC/PRF/5 cells using MTT assay. ELISA has been used to measure HBsAg in the treated cell line supernatants. In addition, real-time PCR analysis was performed to evaluate the expression of HBsAg before and after treatment of Onosma in vitro.

Results: The results showed very low root extract cytotoxicity at concentrations under 8 μg/mL. Tissue culture infectious dose 50 was obtained at 63.78 μg/mL. In a dose-dependent and time-dependent manner, a significantly reduced HBsAg secretion was observed at a concentration of 8 ppm at 12 h post-treatment. The real-time PCR result showed relative decreased HBsAg expression at all doses at 12 h post-treatment time.

Discussion: In this study, we first reported anti-HBsAg activity on an Iranian herbal medicine. Havachoobe root extract was shown to be able to inhibit HBsAg in a dose-dependent and time-dependent manner. We find the extract exerts its inhibitory effect of HBsAg by targeting transcription of HBsAg.

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Havachoobe（Onosma dichroanthum Boiss）根提取物可减少 PLC/PRF/5 细胞系中乙型肝炎病毒表面抗原的分泌。

背景：目前，许多工作都集中在乙型肝炎病毒（HBV）的功能性治疗上。这可以通过抑制 HBV 表面抗原（HBsAg）的分泌来实现。科学界对这方面的天然产品非常感兴趣：使用 Havachoobe（Onosma dichroanthum BoissI）的根提取物评估其抗 HBsAg 分泌的活性。方法：Havachoobe 是在附近的药店购买的，植物根提取物是用水醇法提取的。使用 MTT 法检测提取物对 PLC/PRF/5 细胞的细胞毒活性。酶联免疫吸附法（ELISA）用于检测处理过的细胞系上清液中的 HBsAg。此外，还进行了实时 PCR 分析，以评估体外处理 Onosma 前后 HBsAg 的表达情况：结果表明，当根提取物的浓度低于 8 μg/mL 时，其细胞毒性非常低。组织培养感染剂量 50 为 63.78 μg/mL。在剂量依赖性和时间依赖性的作用下，浓度为 8 ppm 时，处理后 12 小时的 HBsAg 分泌明显减少。实时聚合酶链式反应（real-time PCR）结果显示，在处理后 12 小时，所有剂量的 HBsAg 表达量都相对减少：本研究首次报道了一种伊朗草药的抗 HBsAg 活性。研究表明，Havachoobe 根提取物能够以剂量依赖性和时间依赖性的方式抑制 HBsAg。我们发现该提取物通过靶向 HBsAg 的转录来发挥抑制 HBsAg 的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Intervirology 医学-病毒学

CiteScore

5.40

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： ''Intervirology'' covers progress in both basic and clinical virus research, and aims to provide a forum for the various disciplines within virology. Issues publishing original papers alternate with thematic issues, focusing on clearly defined topics. This thematic concentration serves to make timely reviews, research reports and controversy easily accessible to both specialists in the field and those who want to keep track of the latest developments outside their own area of interest. In addition to original papers, regular issues publish short communications and letters to the editor to provide readers with a forum for the exchange of ideas and comments. The scope encompasses work on the molecular biology of human and animal viruses, including genome organization and regulation, and the structure and function of viral proteins. The pathogenesis, immunology, diagnosis, epidemiology, prophylaxis and therapy of viral diseases are considered.