Identification of Mucor circinelloides antigens recognized by sera from immunocompromised infected mice

IF 1.5 4区生物学 Q4 MYCOLOGY

Revista Iberoamericana De Micologia Pub Date : 2020-07-01 DOI:10.1016/j.riam.2020.07.002

Maialen Areitio , Adela Martin-Vicente , Aitana Arbizu , Aitziber Antoran , Leire Aparicio-Fernandez , Idoia Buldain , Leire Martin-Souto , Aitor Rementeria , Javier Capilla , Fernando L. Hernando , Andoni Ramirez-Garcia

{"title":"Identification of Mucor circinelloides antigens recognized by sera from immunocompromised infected mice","authors":"Maialen Areitio , Adela Martin-Vicente , Aitana Arbizu , Aitziber Antoran , Leire Aparicio-Fernandez , Idoia Buldain , Leire Martin-Souto , Aitor Rementeria , Javier Capilla , Fernando L. Hernando , Andoni Ramirez-Garcia","doi":"10.1016/j.riam.2020.07.002","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p><span><em>Mucor circinelloides</em></span> is an opportunistic fungus capable of causing mucormycosis, a highly aggressive infection of quick spreading. Besides, it also has a high mortality rate due to late diagnosis and difficult treatment.</p></div><div><h3>Aims</h3><p>In this study we have identified the most immunoreactive proteins of the secretome and the total protein extract of <em>M. circinelloides</em> using sera from immunocompromised infected mice.</p></div><div><h3>Methods</h3><p><span>The proteins of the secretome and the total extract were analyzed by two-dimensional electrophoresis and the most immunoreactive antigens were detected by Western Blot, facing the sera of immunocompromised infected mice to the proteins obtained in both extracts of </span><em>M. circinelloides</em>.</p></div><div><h3>Results</h3><p>Seven antigens were detected in the secretome extract, and two in the total extract, all of them corresponding only to three proteins. The enzyme enolase was detected in both extracts, while triosephosphate isomerase was detected in the secretome, and heat shock protein HSS1 in the total extract.</p></div><div><h3>Conclusions</h3><p>In this work the most immunoreactive antigens of the secretome and the total extract of <em>M. circinelloides</em> were identified. The identified proteins are well known fungal antigens and, therefore, these findings can be useful for future research into alternatives for the diagnosis and treatment of mucormycosis.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"37 3","pages":"Pages 81-86"},"PeriodicalIF":1.5000,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.riam.2020.07.002","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revista Iberoamericana De Micologia","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1130140620300322","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MYCOLOGY","Score":null,"Total":0}

引用次数: 4

Abstract

Background

Mucor circinelloides is an opportunistic fungus capable of causing mucormycosis, a highly aggressive infection of quick spreading. Besides, it also has a high mortality rate due to late diagnosis and difficult treatment.

Aims

In this study we have identified the most immunoreactive proteins of the secretome and the total protein extract of M. circinelloides using sera from immunocompromised infected mice.

Methods

The proteins of the secretome and the total extract were analyzed by two-dimensional electrophoresis and the most immunoreactive antigens were detected by Western Blot, facing the sera of immunocompromised infected mice to the proteins obtained in both extracts of M. circinelloides.

Results

Seven antigens were detected in the secretome extract, and two in the total extract, all of them corresponding only to three proteins. The enzyme enolase was detected in both extracts, while triosephosphate isomerase was detected in the secretome, and heat shock protein HSS1 in the total extract.

Conclusions

In this work the most immunoreactive antigens of the secretome and the total extract of M. circinelloides were identified. The identified proteins are well known fungal antigens and, therefore, these findings can be useful for future research into alternatives for the diagnosis and treatment of mucormycosis.

查看原文本刊更多论文

免疫功能低下感染小鼠血清中环状毛霉抗原的鉴定

背景:环形毛霉是一种能够引起毛霉病的机会性真菌，这是一种快速传播的高度侵袭性感染。此外，由于诊断晚，治疗困难，死亡率高。目的在本研究中，我们利用免疫功能低下的感染小鼠血清，鉴定了环状螺旋杆菌分泌组的大部分免疫反应蛋白和总蛋白提取物。方法采用双向电泳分析分泌组蛋白和总提取物蛋白，采用免疫印迹法检测免疫功能低下小鼠血清对两种提取物蛋白的免疫反应性。结果分泌组提取物中检测到7种抗原，总提取物中检测到2种抗原，均仅对应3种蛋白。两种提取物中均检测到烯醇化酶，分泌组中检测到三磷酸异构酶，总提取物中检测到热休克蛋白HSS1。结论本工作鉴定出了麻霉分泌组和总提取物的大部分免疫活性抗原。鉴定的蛋白质是众所周知的真菌抗原，因此，这些发现对未来研究毛霉病的诊断和治疗的替代方法是有用的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Revista Iberoamericana De Micologia MYCOLOGY-

CiteScore

1.90

自引率

0.00%

发文量

审稿时长

81 days

期刊介绍： Revista Iberoamericana de Micología (Ibero-American Journal of Mycology) is the official journal of the Asociación Española de Micología, Asociación Venezolana de Micología and Asociación Argentina de Micología (The Spanish, Venezuelan, and Argentinian Mycology Associations). The Journal gives priority to publishing articles on studies associated with fungi and their pathogenic action on humans and animals, as well as any scientific studies on any aspect of mycology. The Journal also publishes, in Spanish and in English, original articles, reviews, mycology forums, editorials, special articles, notes, and letters to the editor, that have previously gone through a scientific peer review process.