{"title":"A comparison of cell-culture plasticwares for effective human cytokine-induced killer cell culture.","authors":"Panwadee Pluangnooch, Chaiphichit Phayangkhe, Kitipong Soontrapa","doi":"10.12932/AP-140720-0913","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Cytokine-induced killer (CIK) cells are ex-vivo expanded T cells which present a phenotype of both T and Natural Killer cell properties.</p><p><strong>Objective: </strong>To compare the proliferation and functional properties of human CIK cells cultured in three cell culture plasticwares.</p><p><strong>Methods: </strong>The number and viability of CIK cells were monitored. The expression of surface markers (CD3 and CD56), TH1 cytokines (IFN-γ and TNF-α), and cytolytic granules (granzyme B and perforin) were determined by flow cytometry.</p><p><strong>Results: </strong>The number of CIK cells cultured in a static bag was highest compared to those in a petri dish and gas-permeable flask. However, CIK cells cultured in all plasticwares similarity expressed surface marker, TH1 cytokines, and cytolytic granules.</p><p><strong>Conclusions: </strong>Considering safety, efficacy, and cost, a static bag is the best plasticware for culturing CIK cells.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.12932/AP-140720-0913","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
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Abstract
Background: Cytokine-induced killer (CIK) cells are ex-vivo expanded T cells which present a phenotype of both T and Natural Killer cell properties.
Objective: To compare the proliferation and functional properties of human CIK cells cultured in three cell culture plasticwares.
Methods: The number and viability of CIK cells were monitored. The expression of surface markers (CD3 and CD56), TH1 cytokines (IFN-γ and TNF-α), and cytolytic granules (granzyme B and perforin) were determined by flow cytometry.
Results: The number of CIK cells cultured in a static bag was highest compared to those in a petri dish and gas-permeable flask. However, CIK cells cultured in all plasticwares similarity expressed surface marker, TH1 cytokines, and cytolytic granules.
Conclusions: Considering safety, efficacy, and cost, a static bag is the best plasticware for culturing CIK cells.
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