Urinary Apolipoprotein C3 Is a Potential Biomarker for Alzheimer's Disease.

IF 1.4 Q4 CLINICAL NEUROLOGY
Dementia and Geriatric Cognitive Disorders Extra Pub Date : 2020-09-11 eCollection Date: 2020-09-01 DOI:10.1159/000509561
Yumi Watanabe, Yoshitoshi Hirao, Kensaku Kasuga, Takayoshi Tokutake, Kaori Kitamura, Shumpei Niida, Takeshi Ikeuchi, Kazutoshi Nakamura, Tadashi Yamamoto
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引用次数: 14

Abstract

Introduction: Biomarkers of Alzheimer's disease (AD) that can easily be measured in routine health checkups are desirable. Urine is a source of biomarkers that can be collected easily and noninvasively. We previously reported on the comprehensive profile of the urinary proteome of AD patients and identified proteins estimated to be significantly increased or decreased in AD patients by a label-free quantification method. The present study aimed to validate urinary levels of proteins that significantly differed between AD and control samples from our proteomics study (i.e., apolipoprotein C3 [ApoC3], insulin-like growth factor-binding protein 3 [Igfbp3], and apolipoprotein D [ApoD]).

Methods: Enzyme-linked immunosorbent assays (ELISAs) were performed using urine samples from the same patient and control groups analyzed in the previous proteomics study (18 AD and 18 controls, set 1) and urine samples from an independent group of AD patients and controls (13 AD, 5 mild cognitive impairment [MCI], and 32 controls) from the National Center for Geriatrics and Gerontology Biobank (set 2).

Results: In set 1, the crude urinary levels of ApoD, Igfbp3, and creatinine-adjusted ApoD were significantly higher in the AD group relative to the control group (p = 0.003, p = 0.002, and p = 0.019, respectively), consistent with our previous proteomics results. In set 2, however, the crude urinary levels of Igfbp3 were significantly lower in the AD+MCI group than in the control group (p = 0.028), and the levels of ApoD and ApoC3 did not differ significantly compared to the control group. Combined analysis of all samples revealed creatinine-adjusted ApoC3 levels to be significantly higher in the AD+MCI group (p = 0.015) and the AD-only group (p = 0.011) relative to the control group.

Conclusion: ApoC3 may be a potential biomarker for AD, as validated by ELISA. Further analysis of ApoC3 as a urinary biomarker for AD is warranted.

Abstract Image

Abstract Image

Abstract Image

尿载脂蛋白C3是阿尔茨海默病的潜在生物标志物。
简介:阿尔茨海默病(AD)的生物标志物,可以很容易地测量在常规健康检查是可取的。尿液是一种生物标志物的来源,可以很容易和无创地收集。我们之前报道了AD患者尿蛋白质组的综合概况,并通过无标记量化方法确定了AD患者中估计显着增加或减少的蛋白质。本研究旨在验证AD患者尿液中的蛋白质水平与我们蛋白质组学研究的对照样本之间的显著差异(即载脂蛋白C3 [ApoC3],胰岛素样生长因子结合蛋白3 [Igfbp3]和载脂蛋白D [ApoD])。方法:酶联免疫吸附测定(elisa)使用来自先前蛋白质组学研究中分析的同一患者和对照组的尿液样本(18例AD和18例对照,组1)和来自国家老年医学和老年生物学生物库的独立组AD患者和对照组的尿液样本(13例AD, 5例轻度认知障碍[MCI]和32例对照)(组2)。在set 1中,AD组的粗尿ApoD、Igfbp3和肌酐调节ApoD水平明显高于对照组(p = 0.003、p = 0.002和p = 0.019),与我们之前的蛋白质组学结果一致。然而,在第2组中,AD+MCI组的尿中Igfbp3粗水平显著低于对照组(p = 0.028), ApoD和ApoC3的水平与对照组相比无显著差异。所有样本的综合分析显示,相对于对照组,AD+MCI组(p = 0.015)和AD单独组(p = 0.011)的肌酐调节apop3水平显著高于对照组。结论:经ELISA验证,ApoC3可能是AD的潜在生物标志物。进一步分析apo3作为阿尔茨海默病的尿液生物标志物是有必要的。
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来源期刊
Dementia and Geriatric Cognitive Disorders Extra
Dementia and Geriatric Cognitive Disorders Extra Medicine-Psychiatry and Mental Health
CiteScore
4.30
自引率
0.00%
发文量
18
审稿时长
9 weeks
期刊介绍: This open access and online-only journal publishes original articles covering the entire spectrum of cognitive dysfunction such as Alzheimer’s and Parkinson’s disease, Huntington’s chorea and other neurodegenerative diseases. The journal draws from diverse related research disciplines such as psychogeriatrics, neuropsychology, clinical neurology, morphology, physiology, genetic molecular biology, pathology, biochemistry, immunology, pharmacology and pharmaceutics. Strong emphasis is placed on the publication of research findings from animal studies which are complemented by clinical and therapeutic experience to give an overall appreciation of the field. Dementia and Geriatric Cognitive Disorders Extra provides additional contents based on reviewed and accepted submissions to the main journal Dementia and Geriatric Cognitive Disorders Extra .
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