Chikungunya virus infections in Finnish travellers 2009-2019.

Q1 Environmental Science

Infection Ecology and Epidemiology Pub Date : 2020-08-26 DOI:10.1080/20008686.2020.1798096

A J Jääskeläinen, L Kareinen, T Smura, H Kallio-Kokko, O Vapalahti

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引用次数: 2

Abstract

The mosquito-borne chikungunya virus (CHIKV) causes an acute febrile illness with rash, joint and muscle pain.A realtime RT-PCR assay for CHIKV detecting non-structural protein (nsP2; CHIKV nsP2-RT-qPCR) was set up. All the serodiagnosed CHIKV cases detected during 2009-2019 in Finland were screened with the assay, followed by isolations attempts and sequencing using Sanger and next generation sequencing (NGS). To validate the assay external and in-house quality control samples were used and all were correctly identified. Specificity of the assay was 100%. Assay was sensitive to detect CHIKV RNA in dilution of 10^-8.During years 2009-2019 34 patients were diagnosed for acute CHIKV infection. Twelve out of 34 cases were positive by CHIKV nsP2-RT-qPCR.Two CHIKV isolations succeeded from two individuals infected originally in Thailand, 2019. From 12 CHIKV nsP2-RT-qPCR positive samples, five (42%) CHIKVs were successfully sequenced. In this study, CHIKVs from year 2019 clustered with CHIKV ECSA-lineage forming sub-cluster with strains from ones detected in Bangladesh 2017, and the ones from Jamaica (2014) within Asian lineage showing highest similarity to strains detected in Caribbean outbreak 2013-15. Majority of the CHIKV infections detected in Finland originates from Asia and virus lineages reflect the global circulation of the pathogen.

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2009-2019年芬兰旅行者基孔肯雅病毒感染情况。

蚊子传播的基孔肯雅病毒(CHIKV)引起急性发热性疾病，伴有皮疹、关节和肌肉疼痛。CHIKV非结构蛋白(nsP2)的实时RT-PCR检测建立CHIKV nsP2-RT-qPCR)。2009-2019年在芬兰检测到的所有血清诊断的CHIKV病例均采用该方法进行筛查，然后进行分离尝试，并使用Sanger和下一代测序(NGS)进行测序。为了验证分析，使用了外部和内部质量控制样品，并正确识别。特异性为100%。在10-8的稀释条件下，检测到CHIKV RNA的灵敏度较高。2009年至2019年期间，34名患者被诊断为急性CHIKV感染。34例中有12例CHIKV nsP2-RT-qPCR阳性。2019年，从最初在泰国感染的两个人中成功分离出两株CHIKV病毒。从12份CHIKV nsP2-RT-qPCR阳性样本中，成功测序了5份(42%)CHIKV。在本研究中，来自2019年的CHIKV与来自2017年孟加拉国和2014年牙买加的CHIKV形成亚聚类，在亚洲谱系中与2013- 2015年加勒比暴发中检测到的菌株具有最高的相似性。在芬兰发现的大多数CHIKV感染来自亚洲，病毒谱系反映了该病原体的全球传播。

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来源期刊

Infection Ecology and Epidemiology Environmental Science-Environmental Science (miscellaneous)

CiteScore

8.70

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： Infection Ecology & Epidemiology aims to stimulate inter-disciplinary collaborations dealing with a range of subjects, from the plethora of zoonotic infections in humans, over diseases with implication in wildlife ecology, to advanced virology and bacteriology. The journal specifically welcomes papers from studies where researchers from multiple medical and ecological disciplines are collaborating so as to increase our knowledge of the emergence, spread and effect of new and re-emerged infectious diseases in humans, domestic animals and wildlife. Main areas of interest include, but are not limited to: 1.Zoonotic microbioorganisms 2.Vector borne infections 3.Gastrointestinal pathogens 4.Antimicrobial resistance 5.Zoonotic microbioorganisms in changing environment