Cytotoxicity Potential of Endophytic Fungi Extracts from Terminalia catappa against Human Cervical Cancer Cells.

IF 3.4 Q2 TOXICOLOGY
Journal of Toxicology Pub Date : 2020-09-22 eCollection Date: 2020-01-01 DOI:10.1155/2020/8871152
Michèle Stella Majoumouo, Marius Belmondo Tincho, Rufin Marie Kouipou Toghueo, Thureyah Morris, Donavon Charles Hiss, Fabrice Fekam Boyom, Chitra Mandal
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引用次数: 16

Abstract

Endophytic fungi are potential sources of novel bioactive metabolites from a natural product drug discovery perspective. This study reports the bioactivity-directed fractionation of the secondary metabolites of the ethyl acetate extract of a fermentation culture of endophytic fungi from Terminalia catappa which were then evaluated for their cytotoxicity against human cervical cancer (HeLa) cells and human foreskin fibroblast (HFF) cells. Furthermore, apoptosis was determined using the Annexin V/propidium iodide (PI) flow cytometry assay. Endophyte extracts N2, N7, N8, N97, N169, and N233 were obtained from Trichoderma sp, Phoma sp, Phomopsis phyllanticola, Fusarium oxyporum, Collectotrichum sp, and Cryptococcus flavescens, respectively. The N97 extract was most active with a 50% inhibitory concentration (IC50) of 33.35 µg/ml. A 50% cytotoxic concentration (CC50) of 268.4 µg/ml was obtained with HFF cells and the selectivity index (SI) was 8.01. The percentages of cell populations were increased at late apoptosis (Annexin+/PI+), with the percentages of 27.4 ± 0.3 and 19.2 ± 0.01 obtained, respectively, for 50 µg/ml and 80 µg/ml of the N97 extract and 2.1 ± 0.1 obtained for the control in late apoptosis (Annexin V+/PI+) . Moreover, a higher reduction in the percentage of viable cells was observed in the HeLa control cells (93.6 ± 0.3), but the percentages of viable HeLa cells were 37 ± 0.05 and 45 ± 0.1, respectively, for the 50 µg/ml and 80 µg/ml treatments with the N97 extract. Also, the percentages of 34.7 ± 0.1 and 33.9 ± 0.4 were, respectively, obtained for 50 µg/ml and 80 µg/ml compared to the control with 4.6 ± 0.2, in early apoptosis (Annexin V+/PI-). These findings highlight the anticancer potential of the N97 extract of endophytic fungi from Terminalia catappa, which is mediated through apoptosis and presumably also attenuation of chemoresistance.

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刺尾草内生真菌提取物对人宫颈癌细胞的细胞毒性潜力。
从天然产物药物发现的角度来看,内生真菌是新型生物活性代谢物的潜在来源。本研究报道了以生物活性为导向的对刺尾草内生真菌发酵培养物乙酸乙酯提取物次级代谢物的分离,并对其对人宫颈癌(HeLa)细胞和人包皮成纤维细胞(HFF)细胞的细胞毒性进行了评价。此外,采用Annexin V/碘化丙啶(PI)流式细胞术检测细胞凋亡。从木霉(Trichoderma sp)、Phoma sp、phyllanticola Phomopsis sp、oxyporum Fusarium、Collectotrichum sp和隐球菌(Cryptococcus flavescens)中分别获得内生菌提取物N2、N7、N8、N97、N169和N233。N97提取物活性最高,50%抑制浓度(IC50)为33.35µg/ml。HFF细胞的细胞毒浓度(CC50)为268.4µg/ml,选择性指数为8.01。凋亡晚期(Annexin+/PI+)细胞群百分比增加,50µg/ml和80µg/ml N97提取物组细胞群百分比分别为27.4±0.3和19.2±0.01,对照组细胞群百分比分别为2.1±0.1。此外,在HeLa对照细胞中,活细胞百分比下降幅度较大(93.6±0.3),而在N97提取物50µg/ml和80µg/ml处理下,HeLa活细胞百分比分别为37±0.05和45±0.1。在早期凋亡(Annexin V+/PI-)中,50µg/ml和80µg/ml的比例分别为34.7±0.1和33.9±0.4,而对照组为4.6±0.2。这些发现强调了刺麻内生真菌N97提取物的抗癌潜力,这种潜力可能是通过细胞凋亡介导的,也可能是通过抑制化学耐药性来实现的。
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来源期刊
Journal of Toxicology
Journal of Toxicology TOXICOLOGY-
CiteScore
5.50
自引率
3.40%
发文量
0
审稿时长
10 weeks
期刊介绍: Journal of Toxicology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of toxicological sciences. The journal will consider articles looking at the structure, function, and mechanism of agents that are toxic to humans and/or animals, as well as toxicological medicine, risk assessment, safety evaluation, and environmental health.
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