Biochemical Constituent of Ginkgo biloba (Seed) 80% Methanol Extract Inhibits Cholinesterase Enzymes in Javanese Medaka (Oryzias javanicus) Model.

IF 3.4 Q2 TOXICOLOGY
Journal of Toxicology Pub Date : 2020-09-23 eCollection Date: 2020-01-01 DOI:10.1155/2020/8815313
Ibrahim Hassan, Wan Norhamidah Wan Ibrahim, Ferdaus Mohamat Yusuf, Siti Aqlima Ahmad, Syahida Ahmad
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引用次数: 7

Abstract

Background: Pathophysiological changes leading to the death of nerve cells present in the brain and spinal cord are referred to as neurodegenerative diseases. Presently, treatment of these diseases is not effective and encounters many challenges due to the cost of drug and side effects. Thus, the search for the alternative agents to replace synthetic drugs is in high demand. Therefore, the aim of this study is to evaluate the anticholinesterase properties of Ginkgo biloba seed.

Methods: The seed was extracted with 80% methanol. Toxicity studies and evaluation of anticholinesterase activities were carried out in adult Javanese medaka (Oryzias javanicus). Phytochemical study to identify the bioactive lead constituents of the crude extract was also carried out using high performance liquid chromatography (HPLC).

Results: The result shows activities with high significant differences at P < 0.001 between the treated and nontreated groups. A bioactive compound (vitaxin) was identified with the aid of HPLC method.

Conclusion: The presence of bioactive compound vitaxin is among the major secondary metabolites that contribute to increasing activities of this plant extract. High anticholinesterase activities and low toxicity effect of this plant show its benefit to be used as natural medicine or supplements.

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银杏(种子)80%甲醇提取物生化成分对爪哇Medaka (Oryzias javanicus)模型胆碱酯酶的抑制作用
背景:导致大脑和脊髓中存在的神经细胞死亡的病理生理变化被称为神经退行性疾病。目前,由于药物成本和副作用,这些疾病的治疗效果并不好,并且遇到了许多挑战。因此,寻找替代合成药物的替代品的需求很大。因此,本研究的目的是评价银杏种子的抗胆碱酯酶特性。方法:用80%甲醇提取种子。对爪哇稻鳉鱼(Oryzias javanicus)进行了毒力研究和抗胆碱酯酶活性评价。采用高效液相色谱法对粗提物进行植物化学研究,鉴定其生物活性成分。结果:治疗组与未治疗组活性差异显著,P < 0.001。用高效液相色谱法鉴定了其中的一种生物活性化合物(维他素)。结论:生物活性化合物vitaxin的存在是提高该植物提取物活性的主要次生代谢产物之一。该植物抗胆碱酯酶活性高,毒性作用低,可作为天然药物或保健品使用。
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来源期刊
Journal of Toxicology
Journal of Toxicology TOXICOLOGY-
CiteScore
5.50
自引率
3.40%
发文量
0
审稿时长
10 weeks
期刊介绍: Journal of Toxicology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of toxicological sciences. The journal will consider articles looking at the structure, function, and mechanism of agents that are toxic to humans and/or animals, as well as toxicological medicine, risk assessment, safety evaluation, and environmental health.
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