Improvements on marker-free images alignment for electron tomography

IF 3.5 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
C.O.S. Sorzano , F. de Isidro-Gómez , E. Fernández-Giménez , D. Herreros , S. Marco , J.M. Carazo , C. Messaoudi
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引用次数: 5

Abstract

Electron tomography is a technique to obtain three-dimensional structural information of samples. However, the technique is limited by shifts occurring during acquisition that need to be corrected before the reconstruction process. In 2009, we proposed an approach for post-acquisition alignment of tilt series images. This approach was marker-free, based on patch tracking and integrated in free software. Here, we present improvements to the method to make it more reliable, stable and accurate. In addition, we modified the image formation model underlying the alignment procedure to include different deformations occurring during acquisition. We propose a new way to correct these computed deformations to obtain reconstructions with reduced artifacts. The new approach has demonstrated to improve the quality of the final 3D reconstruction, giving access to better defined structures for different transmission electron tomography methods: resin embedded STEM-tomography and cryo-TEM tomography. The method is freely available in TomoJ software.

Abstract Image

电子断层扫描无标记图像对准的改进
电子层析成像是一种获取样品三维结构信息的技术。然而,该技术受到采集过程中发生的移位的限制,这些移位需要在重建过程之前进行纠正。2009年,我们提出了一种倾斜序列图像采集后对齐的方法。这种方法是无标记的,基于补丁跟踪并集成在自由软件中。在此,我们对该方法进行了改进,使其更加可靠、稳定和准确。此外,我们修改了对准过程的图像形成模型,以包括采集过程中发生的不同变形。我们提出了一种新的方法来校正这些计算变形,以获得具有减少伪影的重建。新方法已被证明可以提高最终3D重建的质量,为不同的透射电子断层扫描方法(树脂嵌入stem断层扫描和冷冻tem断层扫描)提供更好的定义结构。该方法在TomoJ软件中免费提供。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Structural Biology: X
Journal of Structural Biology: X Biochemistry, Genetics and Molecular Biology-Structural Biology
CiteScore
6.50
自引率
0.00%
发文量
20
审稿时长
62 days
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