Circadian actions of orexins on the retinorecipient lateral geniculate complex in rat.

The Journal of Physiology Pub Date : 2021-01-01 Epub Date: 2020-10-14 DOI:10.1113/JP280275
Lukasz Chrobok, Jagoda Stanislawa Jeczmien-Lazur, Kamil Pradel, Jasmin Daniela Klich, Monika Bubka, Michal Wojcik, Mariusz Kepczynski, Marian Henryk Lewandowski
{"title":"Circadian actions of orexins on the retinorecipient lateral geniculate complex in rat.","authors":"Lukasz Chrobok,&nbsp;Jagoda Stanislawa Jeczmien-Lazur,&nbsp;Kamil Pradel,&nbsp;Jasmin Daniela Klich,&nbsp;Monika Bubka,&nbsp;Michal Wojcik,&nbsp;Mariusz Kepczynski,&nbsp;Marian Henryk Lewandowski","doi":"10.1113/JP280275","DOIUrl":null,"url":null,"abstract":"<p><strong>Key points: </strong>Rhythmic processes in living organisms are controlled by biological clocks. The orexinergic system of the lateral hypothalamus carries circadian information to provide arousal for the brain during the active phase. Here, we show that orexins exert an excitatory action in three parts of the lateral geniculate nucleus (LGN), in particular upon directly retinorecipient neurons in the non-image forming visual structures. We provide evidence for the high nocturnal levels of orexins with stable circadian expression of predominant orexin receptor 2 in the LGN. Our data additionally establish the convergence of orexinergic and pituitary adenylate cyclase (PAC)-activating peptide/PAC1 receptor systems (used by melanopsin-expressing retinal ganglion cells), which directly regulates responses to the retinal input. These results help us better understand circadian orexinergic control over the non-image forming subcortical visual system, forming the animal's preparedness for the behaviourally active night.</p><p><strong>Abstract: </strong>The orexinergic system of the lateral hypothalamus is tightly interlinked with the master circadian clock and displays daily variation in activity to provide arousal-related excitation for the plethora of brain structures in a circadian manner. Here, using a combination of electrophysiological, optogenetic, histological, molecular and neuronal tracing methods, we explore a particular link between orexinergic and visual systems in rat. The results of the present study demonstrate that orexinergic fibre density at the area of subcortical visual system exerts a clear day to night variability, reaching a maximum at behaviourally active night. We also show pronounced electrophysiological activations of neurons in the lateral geniculate nucleus by orexin A through 24 h, via identified distinct orexin receptors, with the ventrolateral geniculate displaying a daily cycle of responsiveness. In addition, for the first time, we provide a direct evidence for orexins to act on retinorecipient neurons with a high convergence of orexinergic and putatively retinal pituitary adenylate cyclase (PAC)-activating peptide/PAC1 receptor systems. Altogether, the present study ties orexins to non-image forming visual structures with implications for circadian orexinergic modulation of neurons, which process information on ambient light levels.</p>","PeriodicalId":501632,"journal":{"name":"The Journal of Physiology","volume":" ","pages":"231-252"},"PeriodicalIF":0.0000,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1113/JP280275","citationCount":"10","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Journal of Physiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1113/JP280275","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2020/10/14 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 10

Abstract

Key points: Rhythmic processes in living organisms are controlled by biological clocks. The orexinergic system of the lateral hypothalamus carries circadian information to provide arousal for the brain during the active phase. Here, we show that orexins exert an excitatory action in three parts of the lateral geniculate nucleus (LGN), in particular upon directly retinorecipient neurons in the non-image forming visual structures. We provide evidence for the high nocturnal levels of orexins with stable circadian expression of predominant orexin receptor 2 in the LGN. Our data additionally establish the convergence of orexinergic and pituitary adenylate cyclase (PAC)-activating peptide/PAC1 receptor systems (used by melanopsin-expressing retinal ganglion cells), which directly regulates responses to the retinal input. These results help us better understand circadian orexinergic control over the non-image forming subcortical visual system, forming the animal's preparedness for the behaviourally active night.

Abstract: The orexinergic system of the lateral hypothalamus is tightly interlinked with the master circadian clock and displays daily variation in activity to provide arousal-related excitation for the plethora of brain structures in a circadian manner. Here, using a combination of electrophysiological, optogenetic, histological, molecular and neuronal tracing methods, we explore a particular link between orexinergic and visual systems in rat. The results of the present study demonstrate that orexinergic fibre density at the area of subcortical visual system exerts a clear day to night variability, reaching a maximum at behaviourally active night. We also show pronounced electrophysiological activations of neurons in the lateral geniculate nucleus by orexin A through 24 h, via identified distinct orexin receptors, with the ventrolateral geniculate displaying a daily cycle of responsiveness. In addition, for the first time, we provide a direct evidence for orexins to act on retinorecipient neurons with a high convergence of orexinergic and putatively retinal pituitary adenylate cyclase (PAC)-activating peptide/PAC1 receptor systems. Altogether, the present study ties orexins to non-image forming visual structures with implications for circadian orexinergic modulation of neurons, which process information on ambient light levels.

食欲素对大鼠视网膜受体外侧膝状复合体的昼夜作用。
重点:生物体内的节律过程是由生物钟控制的。下丘脑外侧的食欲能系统携带昼夜节律信息,在活动阶段为大脑提供唤醒。在这里,我们发现食欲素在外侧膝状核(LGN)的三个部分发挥兴奋作用,特别是在非图像形成视觉结构中的直接视网膜受体神经元上。我们提供的证据表明,夜间高水平的食欲素与LGN中主要的食欲素受体2的稳定昼夜表达有关。我们的数据还证实了食欲能和垂体腺苷酸环化酶(PAC)激活肽/PAC1受体系统(由表达黑视素的视网膜神经节细胞使用)的会聚性,该系统直接调节对视网膜输入的反应。这些结果有助于我们更好地理解昼夜节律的食欲能控制非图像形成皮层下视觉系统,形成动物对行为活跃的夜晚的准备。摘要:下丘脑外侧的食欲能系统与主生物钟紧密相连,并表现出每日的活动变化,以昼夜节律的方式为过多的大脑结构提供唤醒相关的兴奋。本文结合电生理、光遗传学、组织学、分子和神经元示踪等方法,探讨了大鼠食欲能与视觉系统之间的特殊联系。本研究结果表明,皮层下视觉系统区域的食欲能纤维密度具有明显的昼夜变异性,在行为活跃的夜间达到最大值。我们还发现,通过识别出的不同的食欲素受体,食欲素A在24小时内显著地激活了外侧膝状核神经元的电生理,腹外侧膝状核显示出每日的反应周期。此外,我们首次提供了直接证据,证明食欲素作用于具有食欲能和视网膜垂体腺苷酸环化酶(PAC)激活肽/PAC1受体系统高度收敛的视网膜受体神经元。总的来说,目前的研究将食欲素与非图像形成的视觉结构联系起来,并暗示了神经元的昼夜节律食欲能调节,这些神经元处理环境光照水平的信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信