Isolation, Purification and Antioxidant Activity of The Polysaccharides from Chinese Truffle Tuber sinense.

Abstract

The content of polysaccharides in Tuber sinense was investigated by isolation and purification, followed with the further antioxidant studies in total reducing capacity and radical scavenging activities. The crude extract of polysaccharides was purified by dialysis, column chromatography, and High Performance Liquid Chromatography. The main components of monosaccharide (s) and molecular structure of single polysaccharide were studied by using methylation, GC-MS, and NMR analysis. One new water-soluble non-starch polysaccharide (PTS-A with the yield of 0.41%) from T. sinense was purified and identified on structural characteristics for the first time. The characterizations of PTS-A were studied on physicochemical properties, main components of monosaccharide (s) and molecular structure. PTS-A was identified as glucan, only containing D-glucoses with the molecular structure of [→6) α-D-Glcp (1→6) α-D-Glcp (1→]_n by methylation analysis and NMR. In the determination of total reducing capacity, their reducing abilities could be listed as vitamin C> PTS-A> crude polysaccharides-3> crude polysaccharides-2> crude polysaccharides-1. All of PTS-A, crude polysaccharides-2 and -3 were relatively good scavenger for 1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1- (2,4,6-trinitrophenyl) hydrazyl radicals with the IC₅₀ of 2.81, 4.17 and 3.44 mg/mL, respectively. Thus, the separation and purification of polysaccharides were significant to increase the antioxidant activity in some degree. One new water-soluble 1,6-α-ᴅ-dextran was discovered with the polysaccharide structure identified for the first time. Both PTS-A and crude extracts of polysaccharide performed a potent potential on antioxidant activities. The bioactivities of PTS-A should be generalized to the broader pharmacological effects.