The effects of everyday-life exposure to polycyclic aromatic hydrocarbons on biological age indicators.

Sofia Pavanello, Manuela Campisi, Giuseppe Mastrangelo, Mirjam Hoxha, Valentina Bollati
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引用次数: 22

Abstract

Background: Further knowledge on modifiable aging risk factors is required to mitigate the increasing burden of age-related diseases in a rapidly growing global demographic of elderly individuals. We explored the effect of everyday exposure to polycyclic aromatic hydrocarbons (PAHs), which are fundamental constituents of air pollution, on cellular biological aging. This was determined via the analysis of leukocyte telomere length (LTL), mitochondrial DNA copy number (LmtDNAcn), and by the formation of anti-benzo[a]pyrene diolepoxide (B[a]PDE-DNA) adducts.

Methods: The study population consisted of 585 individuals living in North-East Italy. PAH exposure (diet, indoor activities, outdoor activities, traffic, and residential exposure) and smoking behavior were assessed by questionnaire and anti-B[a]PDE-DNA by high-performance-liquid-chromatography. LTL, LmtDNAcn and genetic polymorphisms [glutathione S-transferase M1 and T1 (GSTM1; GSTT1)] were measured by polymerase chain reaction. Structural equation modelling analysis evaluated these complex relationships.

Results: Anti-B[a]PDE-DNA enhanced with PAH exposure (p = 0.005) and active smoking (p = 0.0001), whereas decreased with detoxifying GSTM1 (p = 0.021) and in females (p = 0.0001). Subsequently, LTL and LmtDNAcn reduced with anti-B[a]PDE-DNA (p = 0.028 and p = 0.018), particularly in males (p = 0.006 and p = 0.0001). Only LTL shortened with age (p = 0.001) while elongated with active smoking (p = 0.0001). Besides this, the most significant determinants of PAH exposure that raised anti-B[a]PDE-DNA were indoor and diet (p = 0.0001), the least was outdoor (p = 0.003).

Conclusion: New findings stemming from our study suggest that certain preventable everyday life exposures to PAHs reduce LTL and LmtDNAcn. In particular, the clear association with indoor activities, diet, and gender opens new perspectives for tailored preventive measures in age-related diseases.

Capsule: Everyday life exposure to polycyclic aromatic hydrocarbons reduces leukocyte telomere length and mitochondrial DNA copy number through anti-B[a]PDE-DNA adduct formation.

Abstract Image

Abstract Image

Abstract Image

日常接触多环芳烃对生物年龄指标的影响。
背景:需要进一步了解可改变的老龄化风险因素,以减轻在快速增长的全球老年人人口中日益增加的年龄相关疾病负担。我们探讨了日常暴露于多环芳烃(PAHs)对细胞生物老化的影响,多环芳烃是空气污染的基本成分。这是通过分析白细胞端粒长度(LTL)、线粒体DNA拷贝数(LmtDNAcn)和抗苯并[a]芘二环氧化物(B[a]PDE-DNA)加合物来确定的。方法:研究人群包括生活在意大利东北部的585人。采用问卷调查和高效液相色谱法检测多环芳烃暴露(饮食、室内活动、室外活动、交通和居住暴露)和吸烟行为。LTL、LmtDNAcn及遗传多态性[谷胱甘肽s转移酶M1和T1 (GSTM1;聚合酶链反应测定GSTT1)。结构方程建模分析评估了这些复杂的关系。结果:抗b [a]PDE-DNA与多环芳烃暴露(p = 0.005)和主动吸烟(p = 0.0001)有关,而与解毒GSTM1 (p = 0.021)和女性(p = 0.0001)有关。随后,LTL和LmtDNAcn随着抗b [a]PDE-DNA而降低(p = 0.028和p = 0.018),尤其是在男性中(p = 0.006和p = 0.0001)。只有LTL随年龄而缩短(p = 0.001),而随积极吸烟而延长(p = 0.0001)。除此之外,多环芳烃暴露导致抗- b [a]PDE-DNA升高的最重要决定因素是室内和饮食(p = 0.0001),室外因素影响最小(p = 0.003)。结论:我们研究的新发现表明,某些可预防的日常生活暴露于多环芳烃会降低LTL和LmtDNAcn。特别是,与室内活动、饮食和性别的明确联系为针对与年龄有关的疾病采取有针对性的预防措施开辟了新的视角。胶囊:日常生活中暴露于多环芳烃通过抗b [a]PDE-DNA加合物的形成减少白细胞端粒长度和线粒体DNA拷贝数。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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