Circular RNA circFANCL motivates the glioma progression via the action on the miR-337-3p/HMGB1 signal axis.

IF 4.7 4区医学 0 MEDICINE, GENERAL & INTERNAL

Minerva medica Pub Date : 2024-12-01 Epub Date: 2020-07-17 DOI:10.23736/S0026-4806.20.06639-2

Tao Wang, Jia Zhou, Mengshi Wu, Wei Luo, Feng Liang

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引用次数: 0

Abstract

Background: Circular RNAs (circRNAs) are significant modulatory molecules in the developing process of glioma. Our study will emphasize on exploring the function of circRNA Fanconi anemia group L protein (circFANCL) and the specific mechanism in glioma.

Methods: The quantitative real-time polymerase chain reaction (qRT-PCR) was implemented for detecting circFANCL, microRNA-337-3p (miR-337-3p) and high-mobility group box-1 (HMGB1). Cell proliferation analysis was assessed using cell counting kit-8 (CCK-8) and colony formation assays. Flow cytometry was applied to determine cell cycle and apoptosis. All protein detection was completed by western blot. Animal experiment was performed to investigate the role of circFANCL in vivo. Dual-luciferase reporter, RNA immunoprecipitation (RIP) and RNA pull-down assays were collectively conducted for analyzing the targeted combination.

Results: CircFANCL was signally increased in glioma tissues and cells, and the up-regulated circFANCL could predict poor prognosis in clinical glioma patients. Down-regulated circFANCL induced the proliferation inhibition, cell cycle arrest and apoptosis promotion of glioma cells in vitro, and inhibited tumor growth in vivo. Regarding the mechanism, circFANCL served as a sponge of miR-337-3p that was a tumor suppressor in glioma and circFANCL targeted miR-337-3p to regulate HMGB1 that was a target gene of miR-337-3p. Furthermore, HMGB1 down-regulation was responsible for the repression of glioma progression caused by knockdown of circFANCL.

Conclusions: Through the illustration of oncogenic function of circFANCL in glioma by the miR-337-3p/HMGB1 axis, we believed that circFANCL might be a great target in the early diagnosis and late treatment of glioma.

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环状 RNA circFANCL 通过对 miR-337-3p/HMGB1 信号轴的作用推动胶质瘤的发展。

背景：环状RNA（circRNA）是胶质瘤发展过程中的重要调节分子。我们的研究将重点探讨循环RNA范可尼贫血L组蛋白（circFANCL）在胶质瘤中的功能及其具体机制：方法：采用实时定量聚合酶链反应（qRT-PCR）检测circFANCL、microRNA-337-3p（miR-337-3p）和高迁移率基团框-1（HMGB1）。细胞增殖分析采用细胞计数试剂盒-8（CCK-8）和集落形成试验进行评估。流式细胞术用于确定细胞周期和细胞凋亡。所有蛋白质检测均通过 Western 印迹法完成。为研究 circFANCL 在体内的作用，进行了动物实验。双荧光素酶报告、RNA免疫沉淀（RIP）和RNA下拉实验共同分析了靶向组合：结果：circFANCL在胶质瘤组织和细胞中明显增高，上调的circFANCL可预测临床胶质瘤患者的不良预后。下调的circFANCL在体外诱导胶质瘤细胞增殖抑制、细胞周期停滞和凋亡，在体内抑制肿瘤生长。在机制方面，circFANCL是胶质瘤抑癌基因miR-337-3p的海绵，circFANCL靶向miR-337-3p调控miR-337-3p的靶基因HMGB1。此外，HMGB1的下调是敲除circFANCL抑制胶质瘤进展的原因：通过miR-337-3p/HMGB1轴对circFANCL在胶质瘤中致癌功能的说明，我们认为circFANCL可能是胶质瘤早期诊断和晚期治疗的一个重要靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Minerva medica 医学-医学：内科

CiteScore

6.40

自引率

6.40%

发文量

358

审稿时长

>12 weeks

期刊介绍： Minerva Medica publishes scientific papers on internal medicine. Manuscripts may be submitted in the form of editorials, original articles, review articles, case reports, special articles, letters to the Editor and guidelines. The journal aims to provide its readers with papers of the highest quality and impact through a process of careful peer review and editorial work. Duties and responsibilities of all the subjects involved in the editorial process are summarized at Publication ethics.