Shape Memory and Osteogenesis Capabilities of the Electrospun Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Modified Poly(l-Lactide) Fibrous Mats.

Tissue Engineering Part A Pub Date : 2021-01-01 Epub Date: 2020-07-28 DOI:10.1089/ten.TEA.2020.0086
Xianliu Wang, Hongyu Yan, Yanbing Shen, Han Tang, Bingcheng Yi, Chunping Qin, Yanzhong Zhang
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引用次数: 20

Abstract

Poly(l-lactide) (PLLA) as one of the most well-known biodegradable polyesters has been studied extensively for bone tissue engineering. If being properly programmed, scaffolds from PLLA can also be endowed with the capability of shape memory. However, several noted issues, for example, mechanical brittleness, high glass transition temperature Tg, and relatively poor shape retention and recovery properties, necessitate modification of the PLLA to improve its application efficacy in physiological conditions. This study is proposed to modify PLLA by having the biodegradable poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) incorporated to form ultrafine composite fibers (i.e., PLLA-PHBV) through electrospinning. Different pairs of PLLA-PHBV at the varying mass ratios of 10:0, 9:1, 8:2, 7:3, 6:4, and 0:10 can be successfully electrospun into fibrous form with the fineness of 2-3 μm. Incorporation of PHBV enables to give rise to desired Tg decreases and also, interestingly, increases in the Young's modulus of the PLLA-PHBV blends, while gradually increasing the PHBV mass ratios up to 30%. The PLLA-PHBV (7:3) formulation is identified to present excellent shape memory properties with high shape fixing ratio (>98%) and shape recovery ratio (>96%) compared to the unmodified PLLA fiber counterpart. Moreover, the PLLA-PHBV (7:3) fibers also show enhanced osteogenesis-inducing ability in the mouse bone mesenchymal stem cells, even under nonosteoinductive conditions. Collectively, for the first time this study demonstrates the enhanced shape memory and osteogenesis capabilities of the electrospun PLLA-PHBV composite fibers, and the researched PLLA-PHBV (7:3) fiber system could be potentially applied as a multifunctional scaffolding material for applications in bone tissue repair and regeneration. Impact statement By first converting the poly(l-lactide) (PLLA)-poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) hybrids into fibrous form at varied mass ratios followed by a thorough characterization, we reasonably demonstrated that incorporation of an appropriate amount of PHBV (i.e., 30%) into the PLLA fibers could give rise to significant improvement on the shape memory capability of the PLLA, along with the desired decreases in the transition temperature (Tg). Moreover, the fibrous PLLA-PHBV (7:3) scaffold was also found to significantly promote the osteogenic commitment in bone mesenchymal stem cells with osteoinductive factors in a synergistic manner. Our biomimicking and shape memory enabled fibrous scaffold of PLLA-PHBV could be used to construct multifunctional three-dimensional scaffold with shape memory effect for bone regeneration.

静电纺丝聚(3-羟基丁酸酯-co-3-羟戊酸酯)改性聚(l-丙交酯)纤维垫的形状记忆和成骨能力。
聚l-丙交酯(PLLA)作为最知名的生物可降解聚酯之一,在骨组织工程中得到了广泛的研究。通过适当的编程,PLLA支架也可以被赋予形状记忆能力。然而,一些值得注意的问题,如机械脆性、高玻璃化转变温度Tg、相对较差的形状保持和恢复性能,需要对PLLA进行改性,以提高其在生理条件下的应用效果。本研究提出将可生物降解的聚(3-羟基丁酸酯-co-3-羟基戊酸酯)(PHBV)掺入静电纺丝形成超细复合纤维(即PLLA-PHBV),对PLLA进行改性。在质量比为10:0、9:1、8:2、7:3、6:4和0:10的情况下,可成功电纺成纤维状,细度为2 ~ 3 μm。PHBV的掺入能够产生所需的Tg降低,有趣的是,pla -PHBV共混物的杨氏模量增加,同时PHBV的质量比逐渐增加到30%。与未改性的PLLA纤维相比,pla - phbv(7:3)配方具有优异的形状记忆性能,具有较高的形状固定率(>98%)和形状恢复率(>96%)。此外,即使在非成骨条件下,pla - phbv(7:3)纤维在小鼠骨间充质干细胞中也显示出增强的成骨诱导能力。总之,本研究首次证明了静电纺pla - phbv复合纤维增强的形状记忆和成骨能力,所研究的pla - phbv(7:3)纤维系统可能作为一种多功能支架材料应用于骨组织修复和再生。通过首先将聚l-丙交酯(PLLA)-聚3-羟基丁酸酯-co-3-羟基戊酸酯(PHBV)以不同的质量比转化成纤维形式,然后进行彻底的表征,我们合理地证明了在PLLA纤维中掺入适量的PHBV(即30%)可以显著提高PLLA的形状记忆能力,同时降低转变温度(Tg)。此外,还发现纤维pla - phbv(7:3)支架与骨诱导因子以协同方式显著促进骨间充质干细胞的成骨承诺。我们制备的具有仿生和形状记忆功能的pla - phbv纤维支架可用于构建具有形状记忆效果的多功能三维骨再生支架。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Tissue Engineering Part A
Tissue Engineering Part A CELL & TISSUE ENGINEERING-BIOTECHNOLOGY & APPLIED MICROBIOLOGY
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