Proliferative Status in the Aqueous Humor of Eyes With Congenital Cataract.

Yinglei Zhang, Dan Li, Qiang Lu, Yu Du, Yi Lu, Xiangjia Zhu
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引用次数: 1

Abstract

Purpose: To measure the concentrations of growth factors in the aqueous humor of patients with congenital cataract and to investigate the biological effects of a selected cytokine (fibroblast growth factor 4 [FGF4]) on cell proliferation, migration, and transformation.

Methods: In the aqueous humor obtained from 55 eyes with congenital cataract and 55 eyes with age-related cataract, 40 growth factors were screened and selected cytokines were confirmed with enzyme-linked immunosorbent assays. After the addition of various concentrations of FGF4 (0, 2.5, 15, or 50 ng/mL) to the incubation medium, cellular functions were evaluated.

Results: The concentration of FGF4 was significantly higher in the aqueous humor of patients with congenital cataract than in that of patients with age-related cataract. The human SRA01/04 lens epithelial cell line was treated with FGF4 and the cell proliferation increased significantly both dose- and time-dependently. The wound healing assay and Transwell migration assay revealed a significant increase in the migration capacity of the SRA01/04 cell line treated with 15 or 50 ng/mL of FGF4 compared with that of control cells. The intensity of immunofluorescent staining for α-smooth muscle actin increased significantly in the SRA01/04 cell line when treated with FGF4. Cytoskeletal protein (F-actin) staining showed that changes of cell morphology were induced in primary lens epithelial cells by FGF4.

Conclusions: This study provides a comprehensive profile of growth factors in congenital cataract. FGF4 induced cellular changes, and may have utility as a biomarker to predict the formation of visual axis opacification. [J Pediatr Ophthalmol Strabismus. 2020;57(3):159-168.].

先天性白内障眼房水内增生状况。
目的:测定先天性白内障患者房水中生长因子的浓度,探讨选定的细胞因子(成纤维细胞生长因子4 [FGF4])对细胞增殖、迁移和转化的生物学作用。方法:从55眼先天性白内障和55眼年龄相关性白内障房水中筛选40种生长因子,并采用酶联免疫吸附法对所选细胞因子进行鉴定。在培养液中加入不同浓度的FGF4(0、2.5、15或50 ng/mL)后,评估细胞功能。结果:先天性白内障房水中FGF4浓度明显高于年龄相关性白内障房水。FGF4作用于人SRA01/04晶状体上皮细胞系后,细胞增殖呈剂量依赖性和时间依赖性。伤口愈合实验和Transwell迁移实验显示,与对照细胞相比,15或50 ng/mL FGF4处理的SRA01/04细胞株的迁移能力显著增加。FGF4对SRA01/04细胞α-平滑肌肌动蛋白的免疫荧光染色强度显著升高。细胞骨架蛋白(F-actin)染色显示FGF4诱导原代晶状体上皮细胞形态发生改变。结论:本研究提供了先天性白内障生长因子的全面概况。FGF4诱导细胞变化,并可能作为一种生物标志物来预测视觉轴混浊的形成。[J].儿童眼斜视,2020;57(3):159-168。
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