Hypoxia regulates human mast cell adhesion to fibronectin via the PI3K/AKT signaling pathway.

IF 3.3 3区 生物学 Q3 CELL BIOLOGY
Joanna Pastwińska, Aurelia Walczak-Drzewiecka, Magdalena Łukasiak, Marcin Ratajewski, Jarosław Dastych
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引用次数: 3

Abstract

A decrease in oxygen concentration is a hallmark of inflammatory reactions resulting from infection or homeostasis disorders. Mast cells interact with extracellular matrix and other cells by adhesion receptors. We investigated the effect of hypoxia on integrin-mediated mast cell adhesion to fibronectin. We found that it was mediated by the α5/β1 receptor and that hypoxia significantly upregulated this process. Hypoxia-mediated increases in mast cell adhesion occurred without increased surface expression of integrins, suggesting regulation by inside-out integrin signaling. Hypoxia also mediated an increase in phosphorylation of Akt, and PI3'kinase inhibitors abolished hypoxia-mediated mast cell adhesion. Hypoxia upregulates the function of integrin receptors by PI3' kinase-dependent signaling. This process might be important for the location of mast cells at inflammatory sites.

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Abstract Image

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缺氧通过PI3K/AKT信号通路调节人肥大细胞对纤维连接蛋白的粘附。
氧浓度降低是由感染或体内平衡紊乱引起的炎症反应的标志。肥大细胞通过粘附受体与细胞外基质及其他细胞相互作用。我们研究了缺氧对整合素介导的肥大细胞对纤维连接蛋白粘附的影响。我们发现这是由α5/β1受体介导的,而缺氧显著上调了这一过程。缺氧介导的肥大细胞黏附增加发生在没有增加表面整合素表达的情况下,表明由内向外整合素信号传导调节。缺氧也介导Akt磷酸化的增加,pi3激酶抑制剂可消除缺氧介导的肥大细胞粘附。缺氧通过PI3激酶依赖性信号通路上调整合素受体的功能。这个过程可能对肥大细胞在炎症部位的定位很重要。
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来源期刊
CiteScore
6.40
自引率
0.00%
发文量
7
审稿时长
53 weeks
期刊介绍: Cell Adhesion & Migration is a multi-disciplinary, peer reviewed open access journal that focuses on the biological or pathological implications of cell-cell and cell-microenvironment interactions. The main focus of this journal is fundamental science. The journal strives to serve a broad readership by regularly publishing review articles covering specific disciplines within the field, and by publishing focused issues that provide an overview on specific topics of interest within the field. Cell Adhesion & Migration publishes relevant and timely original research, as well as authoritative overviews, commentaries, and perspectives, providing context for the work presented in Cell Adhesion & Migration and for key results published elsewhere. Original research papers may cover all topics important in the field of cell-cell and cell-matrix interactions. Cell Adhesion & Migration also publishes articles related to cell biomechanics, biomaterial, and development of related imaging technologies.
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