Identification of Tibetan medicine Dida based on DNA barcoding.

Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2020-05-01 Epub Date: 2020-04-01 DOI:10.1080/24701394.2020.1741563

Ruoshi Li, Shuixian Li, Xiaoquan Yang, Yongqiang Guo, Baozhong Duan, Li Xu, Conglong Xia

{"title":"Identification of Tibetan medicine Dida based on DNA barcoding.","authors":"Ruoshi Li, Shuixian Li, Xiaoquan Yang, Yongqiang Guo, Baozhong Duan, Li Xu, Conglong Xia","doi":"10.1080/24701394.2020.1741563","DOIUrl":null,"url":null,"abstract":"The purpose of this study was to test the ability of DNA barcoding to identify the herbal raw trade of Tibetan medicine Dida in China. A reference database for plant-material DNA barcodes was successfully constructed and used to identify 36 commercially samples of Dida collected from Southwest China. The ITS sequence was amplified from these samples and the efficiency of the PCR amplification of ITS was 100%. The DNA sequencing results revealed that 3 samples (8.3%) were authenticated as Swertia chirayita, 2 sequences (5.6%) were authenticated as Swertia mussotii, 3 sequences (8.3%) were authenticated as Swertia ciliata, as recorded in the Tibetan Pharmacopeia. The other samples were authenticated as adulterants and all of them originated from common plants belonging to Saxifraga, Swertia and Halenia. This result indicates Dida pieces that are available in the market have complex origins and may indicate a potential safety issue and DNA barcoding is a convenient tool for market supervision.","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":"31 4","pages":"131-138"},"PeriodicalIF":0.0000,"publicationDate":"2020-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/24701394.2020.1741563","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/24701394.2020.1741563","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2020/4/1 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 1

Abstract

The purpose of this study was to test the ability of DNA barcoding to identify the herbal raw trade of Tibetan medicine Dida in China. A reference database for plant-material DNA barcodes was successfully constructed and used to identify 36 commercially samples of Dida collected from Southwest China. The ITS sequence was amplified from these samples and the efficiency of the PCR amplification of ITS was 100%. The DNA sequencing results revealed that 3 samples (8.3%) were authenticated as Swertia chirayita, 2 sequences (5.6%) were authenticated as Swertia mussotii, 3 sequences (8.3%) were authenticated as Swertia ciliata, as recorded in the Tibetan Pharmacopeia. The other samples were authenticated as adulterants and all of them originated from common plants belonging to Saxifraga, Swertia and Halenia. This result indicates Dida pieces that are available in the market have complex origins and may indicate a potential safety issue and DNA barcoding is a convenient tool for market supervision.

查看原文本刊更多论文

基于DNA条形码的藏药迪达鉴定。

本研究的目的是测试DNA条形码识别藏药迪达在中国药材原料贸易的能力。成功建立了植物材料DNA条形码参考数据库，并对36份来自中国西南地区的迪达商业样品进行了鉴定。从这些样品中扩增ITS序列，其PCR扩增效率为100%。DNA测序结果显示，3个(8.3%)序列鉴定为藏药典记载的chirayita獐牙菜，2个(5.6%)序列鉴定为mussotii獐牙菜，3个(8.3%)序列鉴定为ciliata獐牙菜。其余样品均经鉴定为掺假，均来源于獐牙菜属(Saxifraga)、獐牙菜属(Swertia)和獐牙菜属(Halenia)的常见植物。这一结果表明，市场上出售的迪达碎片来源复杂，可能存在潜在的安全问题，DNA条形码是一种方便的市场监管工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis

自引率

0.00%

发文量