Fast pH-mediated changes of the viscosity of protein solutions studied with a voltage-modulated quartz crystal microbalance.

IF 2.1 4区医学 Q2 Physics and Astronomy

Biointerphases Pub Date : 2020-03-24 DOI:10.1116/1.5140619

Alexander Gödde, Christian Leppin, Frederick S Meyer, Arne Langhoff, Josef Hartl, Patrick Garidel, Diethelm Johannsmann

引用次数: 2

Abstract

An electrochemical quartz crystal microbalance is described, which achieves a time resolution down to 100 μs. Accumulation and averaging over a few hours bring the noise down to about 30 mHz. The application examples are pH-driven viscosity changes in albumin solutions. The pH was switched with the electrode potential. The characteristic response time is in the millisecond range. The focus is on experimental aspects as well as advantages and limitations of the technique.

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用电压调制石英晶体微天平研究蛋白质溶液粘度的快速ph介导变化。

介绍了一种电化学石英晶体微天平，其时间分辨率可达100 μs。几个小时的累积和平均使噪声降低到约30兆赫兹。应用实例是白蛋白溶液中ph值驱动的粘度变化。pH值随电极电位的变化而变化。特征响应时间在毫秒范围内。重点是实验方面以及该技术的优点和局限性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biointerphases BIOPHYSICS-MATERIALS SCIENCE, BIOMATERIALS

CiteScore

4.10

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Biointerphases emphasizes quantitative characterization of biomaterials and biological interfaces. As an interdisciplinary journal, a strong foundation of chemistry, physics, biology, engineering, theory, and/or modelling is incorporated into originated articles, reviews, and opinionated essays. In addition to regular submissions, the journal regularly features In Focus sections, targeted on specific topics and edited by experts in the field. Biointerphases is an international journal with excellence in scientific peer-review. Biointerphases is indexed in PubMed and the Science Citation Index (Clarivate Analytics). Accepted papers appear online immediately after proof processing and are uploaded to key citation sources daily. The journal is based on a mixed subscription and open-access model: Typically, authors can publish without any page charges but if the authors wish to publish open access, they can do so for a modest fee. Topics include: bio-surface modification nano-bio interface protein-surface interactions cell-surface interactions in vivo and in vitro systems biofilms / biofouling biosensors / biodiagnostics bio on a chip coatings interface spectroscopy biotribology / biorheology molecular recognition ambient diagnostic methods interface modelling adhesion phenomena.