Methyl TROSY spectroscopy: A versatile NMR approach to study challenging biological systems

IF 7.3 2区化学 Q2 CHEMISTRY, PHYSICAL

Progress in Nuclear Magnetic Resonance Spectroscopy Pub Date : 2020-02-01 DOI:10.1016/j.pnmrs.2019.09.004

Stefan Schütz, Remco Sprangers

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引用次数: 72

Abstract

A major goal in structural biology is to unravel how molecular machines function in detail. To that end, solution-state NMR spectroscopy is ideally suited as it is able to study biological assemblies in a near natural environment. Based on methyl TROSY methods, it is now possible to record high-quality data on complexes that are far over 100 kDa in molecular weight. In this review, we discuss the theoretical background of methyl TROSY spectroscopy, the information that can be extracted from methyl TROSY spectra and approaches that can be used to assign methyl resonances in large complexes. In addition, we touch upon insights that have been obtained for a number of challenging biological systems, including the 20S proteasome, the RNA exosome, molecular chaperones and G-protein-coupled receptors. We anticipate that methyl TROSY methods will be increasingly important in modern structural biology approaches, where information regarding static structures is complemented with insights into conformational changes and dynamic intermolecular interactions.

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甲基TROSY光谱:一种通用的核磁共振方法来研究具有挑战性的生物系统

结构生物学的一个主要目标是揭示分子机器的详细功能。为此，溶液态核磁共振波谱是非常适合的，因为它能够在接近自然的环境中研究生物组装。基于甲基TROSY方法，现在可以记录分子量远超过100 kDa的复合物的高质量数据。本文综述了甲基TROSY光谱的理论背景，从甲基TROSY光谱中可以提取的信息，以及在大型配合物中用于分配甲基共振的方法。此外，我们还触及了一些具有挑战性的生物系统的见解，包括20S蛋白酶体，RNA外泌体，分子伴侣和g蛋白偶联受体。我们预计甲基TROSY方法将在现代结构生物学方法中越来越重要，其中关于静态结构的信息与构象变化和动态分子间相互作用的见解相辅相成。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Progress in Nuclear Magnetic Resonance Spectroscopy 物理-光谱学

CiteScore

14.30

自引率

8.20%

发文量

审稿时长

62 days

期刊介绍： Progress in Nuclear Magnetic Resonance Spectroscopy publishes review papers describing research related to the theory and application of NMR spectroscopy. This technique is widely applied in chemistry, physics, biochemistry and materials science, and also in many areas of biology and medicine. The journal publishes review articles covering applications in all of these and in related subjects, as well as in-depth treatments of the fundamental theory of and instrumental developments in NMR spectroscopy.