Viscoelastic characterization of diabetic and non-diabetic human adipose tissue.

IF 1 4区医学 Q4 BIOPHYSICS

Biorheology Pub Date : 2020-01-01 DOI:10.3233/BIR-190234

Benjamin A Juliar, Clarissa Strieder-Barboza, Monita Karmakar, Carmen G Flesher, Nicki A Baker, Oliver A Varban, Carey N Lumeng, Andrew J Putnam, Robert W O'Rourke

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引用次数: 8

Abstract

Background: Obesity-induced chronic inflammation and fibrosis in adipose tissue contributes to the progression of type 2 diabetes mellitus (DM). While fibrosis is known to induce mechanical stiffening of numerous tissue types, it is unknown whether DM is associated with alterations in adipose tissue mechanical properties.

Objective: The purpose of this study was to investigate whether DM is associated with differences in bulk viscoelastic properties of adipose tissue from diabetic (DM) and non-diabetic (NDM) obese subjects.

Methods: Bulk shear rheology was performed on visceral (VAT) and subcutaneous (SAT) adipose tissue, collected from obese subjects undergoing elective bariatric surgery. Rheology was also performed on the remaining extracellular matrix (ECM) from decellularized VAT (VAT ECM). Linear mixed models were used to assess whether correlations existed between adipose tissue mechanical properties and DM status, sex, age, and body mass index (BMI).

Results: DM was not associated with significant differences in adipose tissue viscoelastic properties for any of the tissue types investigated. Tissue type dependent differences were however detected, with VAT having significantly lower shear storage and loss moduli than SAT and VAT ECM independent of DM status.

Conclusion: Although DM is typically associated with adipose tissue fibrosis, it is not associated with differences in macroscopic adipose tissue mechanical properties.

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糖尿病和非糖尿病人脂肪组织的粘弹性特性。

背景:肥胖引起的脂肪组织慢性炎症和纤维化有助于2型糖尿病(DM)的进展。虽然已知纤维化可诱导许多组织类型的机械硬化，但尚不清楚糖尿病是否与脂肪组织机械特性的改变有关。目的:本研究的目的是探讨糖尿病是否与糖尿病(DM)和非糖尿病(NDM)肥胖受试者脂肪组织的总体粘弹性特性差异有关。方法:对接受选择性减肥手术的肥胖患者的内脏(VAT)和皮下(SAT)脂肪组织进行体积剪切流变学研究。还对脱细胞增值税(VAT ECM)中剩余的细胞外基质(ECM)进行了流变学研究。使用线性混合模型来评估脂肪组织力学特性与糖尿病状态、性别、年龄和体重指数(BMI)之间是否存在相关性。结果:DM与任何被调查组织类型的脂肪组织粘弹性特性的显著差异无关。然而，检测到组织类型依赖的差异，VAT的剪切储存和损失模量明显低于SAT和VAT ECM，与DM状态无关。结论:虽然DM通常与脂肪组织纤维化相关，但与宏观脂肪组织力学特性的差异无关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biorheology 医学-工程：生物医学

CiteScore

2.00

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Biorheology is an international interdisciplinary journal that publishes research on the deformation and flow properties of biological systems or materials. It is the aim of the editors and publishers of Biorheology to bring together contributions from those working in various fields of biorheological research from all over the world. A diverse editorial board with broad international representation provides guidance and expertise in wide-ranging applications of rheological methods to biological systems and materials. The scope of papers solicited by Biorheology extends to systems at different levels of organization that have never been studied before, or, if studied previously, have either never been analyzed in terms of their rheological properties or have not been studied from the point of view of the rheological matching between their structural and functional properties. This biorheological approach applies in particular to molecular studies where changes of physical properties and conformation are investigated without reference to how the process actually takes place, how the forces generated are matched to the properties of the structures and environment concerned, proper time scales, or what structures or strength of structures are required.