Thymol alleviates AGEs-induced podocyte injury by a pleiotropic effect via NF-κB-mediated by RhoA/ROCK signalling pathway.

IF 3.3 3区生物学 Q3 CELL BIOLOGY

Cell Adhesion & Migration Pub Date : 2020-12-01 DOI:10.1080/19336918.2020.1721172

Qinglian Wang, Zhenwei Shen, Guanghui Qi, Yanfang Zhao, Hongge Zhang, Rong Wang

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引用次数: 8

Abstract

Advanced glycation end products (AGE) are those of the most powerful pathogenic factors that related to diabetic complications. In our study, we investigated the beneficial effects of thymol on AGE induced cell injury and apoptosis in human podocytes (HPCs) and attempted to clarify its mechanisms. Our results revealed that stimulation with AGE could significantly activate RhoA/NF-κB pathway. Results showed thymol could markedly suppress inflammatory responses, cell apoptosis and disordered cytoskeleton. Also thymol restored the expression of podocin, restrained migration capacity. Western blot analysis indicated that it could restore the expression of RhoA, ROCK and vimentin, nephrin, podocin and p65 and IκBα phosphorylation. Moreover, si-RhoA also suppressed the expression of pro-inflammatory cytokines, ROCK, and vimentin and the phosphorylation of p65 and IκBα. In conclusion, thymol inhibits AGE-induced cell injury in HPCs by suppressing the RhoA-NF-κB pathway and may be apromising therapeutic agent.

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百里香酚通过NF-κ b介导的RhoA/ROCK信号通路，通过多效性作用减轻age诱导的足细胞损伤。

晚期糖基化终产物(AGE)是与糖尿病并发症相关的最强大的致病因素。在我们的研究中，我们研究了百里香酚对AGE诱导的人足细胞(HPCs)损伤和凋亡的有益作用，并试图阐明其机制。结果表明，AGE刺激可显著激活RhoA/NF-κB通路。结果表明，百里香酚能明显抑制炎症反应、细胞凋亡和细胞骨架紊乱。百里香酚恢复足docin的表达，抑制迁移能力。Western blot分析显示，它能恢复RhoA、ROCK、vimentin、nephrin、podocin、p65的表达和i - κ b α磷酸化。此外，si-RhoA还抑制促炎细胞因子、ROCK和vimentin的表达以及p65和i- κ b α的磷酸化。结论:百里香酚通过抑制RhoA-NF-κB通路抑制age诱导的HPCs细胞损伤，可能是一种有前景的治疗药物。

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来源期刊

Cell Adhesion & Migration CELL BIOLOGY-

CiteScore

6.40

自引率

0.00%

发文量

审稿时长

53 weeks

期刊介绍： Cell Adhesion & Migration is a multi-disciplinary, peer reviewed open access journal that focuses on the biological or pathological implications of cell-cell and cell-microenvironment interactions. The main focus of this journal is fundamental science. The journal strives to serve a broad readership by regularly publishing review articles covering specific disciplines within the field, and by publishing focused issues that provide an overview on specific topics of interest within the field. Cell Adhesion & Migration publishes relevant and timely original research, as well as authoritative overviews, commentaries, and perspectives, providing context for the work presented in Cell Adhesion & Migration and for key results published elsewhere. Original research papers may cover all topics important in the field of cell-cell and cell-matrix interactions. Cell Adhesion & Migration also publishes articles related to cell biomechanics, biomaterial, and development of related imaging technologies.