Metabolic engineering of Escherichia coli for the utilization of ethanol.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2020-01-21 eCollection Date: 2020-12-01 DOI:10.1186/s40709-020-0111-0

Yujin Cao, Hui Mu, Jing Guo, Hui Liu, Rubing Zhang, Wei Liu, Mo Xian, Huizhou Liu

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Abstract

Background: The fuel ethanol industry has made tremendous progress in the last decades. Ethanol can be obtained by fermentation using a variety of biomass materials as the feedstocks. However, few studies have been conducted on ethanol utilization by microorganisms. The price of petroleum-derived ethanol, easily made by the hydrolysis of ethylene, is even lower than that of bioethanol. If ethanol can be metabolized by microorganisms to produce value-added chemicals, it will open a new door for the utilization of inexpensive ethanol resources.

Results: We constructed an engineered Escherichia coli strain which could utilize ethanol as the sole carbon source. The alcohol dehydrogenase and aldehyde dehydrogenase from Aspergillus nidulans was introduced into E. coli and the recombinant strain acquired the ability to grow on ethanol. Cell growth continued when ethanol was supplied after glucose starvation and 2.24 g L^-1 of ethanol was further consumed during the shake-flasks fermentation process. Then ethanol was further used for the production of mevalonic acid by heterologously expressing its biosynthetic pathway. Deuterium-labeled ethanol-D6 as the feedstock confirmed that mevalonic acid was synthesized from ethanol.

Conclusions: This study demonstrated the possibility of using ethanol as the carbon source by engineered E. coli strains. It can serve as the basis for the construction of more robust strains in the future though the catabolic capacity of ethanol should be further improved.

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利用乙醇的大肠杆菌代谢工程。

背景：过去几十年来，燃料乙醇行业取得了巨大进步。乙醇可以利用各种生物质材料作为原料，通过发酵获得。然而，有关微生物利用乙醇的研究却很少。通过乙烯水解就能轻松制得的石油衍生乙醇的价格甚至低于生物乙醇。如果乙醇能通过微生物代谢产生增值化学品，将为利用廉价乙醇资源打开一扇新的大门：结果：我们构建了一株可利用乙醇作为唯一碳源的工程大肠杆菌。将黑曲霉中的醇脱氢酶和醛脱氢酶引入大肠杆菌，重组菌株获得了以乙醇为唯一碳源的生长能力。在葡萄糖饥饿后提供乙醇时，细胞继续生长，在摇床发酵过程中进一步消耗了 2.24 g L-1 的乙醇。然后，通过异源表达甲羟戊酸的生物合成途径，乙醇被进一步用于生产甲羟戊酸。氘标记的乙醇-D6作为原料证实了甲羟戊酸是由乙醇合成的：本研究证明了工程大肠杆菌菌株使用乙醇作为碳源的可能性。结论：本研究证明了大肠杆菌工程菌株利用乙醇作为碳源的可能性，为今后构建更强大的菌株奠定了基础，但乙醇的分解能力有待进一步提高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.