Subcellular Organelle Toxicity Caused by Arsenic Nanoparticles in Isolated Rat Hepatocytes.

Q1 Medicine

International Journal of Occupational and Environmental Medicine Pub Date : 2020-01-01 DOI:10.15171/ijoem.2020.1614

Rashid Jahangirnejad, Mehdi Goudarzi, Heibatullah Kalantari, Hossein Najafzadeh, Mohsen Rezaei

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Abstract

Background: Arsenic, an environmental pollutant, is a carcinogenic metalloid and also an anticancer agent.

Objective: To evaluate the toxicity of arsenic nanoparticles in rat hepatocytes.

Methods: Freshly isolated rat hepatocytes were exposed to 0, 20, 40, and 100 μM of arsenic nanoparticles and its bulk counterpart. Their viability, reactive oxygen species level, glutathione depletion, mitochondrial and lysosomal damage, and apoptosis were evaluated.

Results: By all concentrations, lysosomal damage and apoptosis were clearly evident in hepatocytes exposed to arsenic nanoparticles. Evaluation of mitochondria and lysosomes revealed that lysosomes were highly damaged.

Conclusion: Exposure to arsenic nanoparticles causes apoptosis and organelle impairment. The nanoparticles have potentially higher toxicity than the bulk arsenic. Lysosomes are highly affected. It seems that, instead of mitochondria, lysosomes are the first target organelles involved in the toxicity induced by arsenic nanoparticles.

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砷纳米颗粒在离体大鼠肝细胞中引起的亚细胞器毒性

背景：砷是一种环境污染物，是一种致癌的类金属，也是一种抗癌剂：评估砷纳米颗粒对大鼠肝细胞的毒性：方法：将新鲜分离的大鼠肝细胞暴露于 0、20、40 和 100 μM 的纳米砷颗粒及其散装颗粒。评估了它们的活力、活性氧水平、谷胱甘肽消耗、线粒体和溶酶体损伤以及细胞凋亡：结果：在所有浓度下，暴露于纳米砷颗粒的肝细胞都明显出现溶酶体损伤和细胞凋亡。对线粒体和溶酶体的评估显示，溶酶体受到严重破坏：结论：暴露于纳米砷颗粒会导致细胞凋亡和细胞器受损。结论：接触纳米砷颗粒会导致细胞凋亡和细胞器受损。溶酶体受到严重影响。看来，溶酶体而不是线粒体是纳米砷诱导毒性的第一个目标细胞器。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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