Molecular types, virulence profiles and antimicrobial resistance of Escherichia coli causing bovine mastitis.

IF 1.3 Q2 VETERINARY SCIENCES
Veterinary Record Open Pub Date : 2019-11-17 eCollection Date: 2019-01-01 DOI:10.1136/vetreco-2019-000369
Magdalena Nüesch-Inderbinen, Nadine Käppeli, Marina Morach, Corinne Eicher, Sabrina Corti, Roger Stephan
{"title":"Molecular types, virulence profiles and antimicrobial resistance of <i>Escherichia coli</i> causing bovine mastitis.","authors":"Magdalena Nüesch-Inderbinen,&nbsp;Nadine Käppeli,&nbsp;Marina Morach,&nbsp;Corinne Eicher,&nbsp;Sabrina Corti,&nbsp;Roger Stephan","doi":"10.1136/vetreco-2019-000369","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong><i>Escherichia coli</i> is an important aetiological agent of bovine mastitis worldwide.</p><p><strong>Methods: </strong>In this study, 82 <i>E. coli</i> from bovine mastitis milk samples from 49 farms were analysed for their genetic diversity using phylogenetic grouping and multilocus sequence typing. The isolates were examined by PCR for a selection of virulence factors (VFs). Antimicrobial susceptibility profiles were assessed using the disk diffusion method.</p><p><strong>Results: </strong>The most prevalent phylogroups were group B1 (41.5 per cent of the isolates) and group A (30.5 per cent). A variety of 35 different sequence types (STs) were identified, including ST1125 (11 per cent), ST58 (9.8 per cent), ST10 (8.5 per cent) and ST88 (7.3 per cent). Aggregate VF scores (the number of unique VFs detected for each isolate) ranged from 1 to 3 for 63.4 per cent of the isolates and were at least 4 for 12.2 per cent. For 24.4 per cent of the isolates, the score was 0. The three most frequent VFs were <i>traT</i>, <i>fyuA</i> and <i>iutA</i>. The majority (72 per cent) of the isolates harboured <i>traT</i>. The majority (68.3 per cent) of the isolates were fully susceptible to all antimicrobials tested, with 22 per cent resistant to ampicillin and 14.6 per cent to tetracycline. Resistance rates were low for gentamicin (3.7 per cent), amoxicillin/clavulanic acid (2.4 per cent) and ceftiofur (1.2 per cent), respectively.</p><p><strong>Conclusion: </strong>Among the study's sample population, <i>E. coli</i> strains were genotypically diverse, even in cows from the same farm, although some STs occurred more frequently than others. Susceptibility to clinically relevant compounds remained high.</p>","PeriodicalId":23565,"journal":{"name":"Veterinary Record Open","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2019-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1136/vetreco-2019-000369","citationCount":"23","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary Record Open","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1136/vetreco-2019-000369","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2019/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 23

Abstract

Background: Escherichia coli is an important aetiological agent of bovine mastitis worldwide.

Methods: In this study, 82 E. coli from bovine mastitis milk samples from 49 farms were analysed for their genetic diversity using phylogenetic grouping and multilocus sequence typing. The isolates were examined by PCR for a selection of virulence factors (VFs). Antimicrobial susceptibility profiles were assessed using the disk diffusion method.

Results: The most prevalent phylogroups were group B1 (41.5 per cent of the isolates) and group A (30.5 per cent). A variety of 35 different sequence types (STs) were identified, including ST1125 (11 per cent), ST58 (9.8 per cent), ST10 (8.5 per cent) and ST88 (7.3 per cent). Aggregate VF scores (the number of unique VFs detected for each isolate) ranged from 1 to 3 for 63.4 per cent of the isolates and were at least 4 for 12.2 per cent. For 24.4 per cent of the isolates, the score was 0. The three most frequent VFs were traT, fyuA and iutA. The majority (72 per cent) of the isolates harboured traT. The majority (68.3 per cent) of the isolates were fully susceptible to all antimicrobials tested, with 22 per cent resistant to ampicillin and 14.6 per cent to tetracycline. Resistance rates were low for gentamicin (3.7 per cent), amoxicillin/clavulanic acid (2.4 per cent) and ceftiofur (1.2 per cent), respectively.

Conclusion: Among the study's sample population, E. coli strains were genotypically diverse, even in cows from the same farm, although some STs occurred more frequently than others. Susceptibility to clinically relevant compounds remained high.

Abstract Image

Abstract Image

引起牛乳腺炎的大肠杆菌的分子类型、毒力特征和抗菌素耐药性。
背景:大肠杆菌是世界范围内牛乳腺炎的重要病原。方法:采用系统发育分组和多位点序列分型方法,对49个牛场乳腺炎乳样品中的82株大肠杆菌进行遗传多样性分析。用PCR检测分离株的毒力因子(VFs)。采用纸片扩散法评价药敏谱。结果:最常见的种群为B1组(41.5%)和A组(30.5%)。共鉴定出35种不同的序列类型,包括ST1125(11%)、ST58(9.8%)、ST10(8.5%)和ST88(7.3%)。63.4%的分离株的总VF分数(每个分离株检测到的唯一VF的数量)在1到3之间,12.2%的分离株至少为4。24.4%的分离株的得分为0。三种最常见的VFs是traT、fyuA和iutA。大多数(72%)的分离株含有traT。大多数(68.3%)分离株对所有测试的抗菌素完全敏感,其中22%对氨苄西林耐药,14.6%对四环素耐药。庆大霉素(3.7%)、阿莫西林/克拉维酸(2.4%)和头孢替福(1.2%)的耐药率较低。结论:在该研究的样本群体中,大肠杆菌菌株具有典型的基因多样性,即使在同一农场的奶牛中也是如此,尽管一些STs比其他STs发生得更频繁。对临床相关化合物的易感性仍然很高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Veterinary Record Open
Veterinary Record Open VETERINARY SCIENCES-
CiteScore
3.00
自引率
0.00%
发文量
25
审稿时长
19 weeks
期刊介绍: Veterinary Record Open is a journal dedicated to publishing specialist veterinary research across a range of topic areas including those of a more niche and specialist nature to that considered in the weekly Vet Record. Research from all disciplines of veterinary interest will be considered. It is an Open Access journal of the British Veterinary Association.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信