Genetic analysis of Taishu horses on and off Tsushima Island: Implications for conservation.

Q3 Veterinary
Journal of Equine Science Pub Date : 2019-07-01 Epub Date: 2019-07-03 DOI:10.1294/jes.30.33
Ayumi Tezuka, Masaki Takasu, Teruaki Tozaki, Atsushi J Nagano
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引用次数: 3

Abstract

Taishu horses are a native Japanese breed, of which only 41 individuals remained on Tsushima Island in 2018. Their genetic diversity is considered lower than that of other Japanese native horse breeds; thus, it needs to be investigated for sustainable conservation of this breed. Historical records revealed that several Taishu individuals were released areas off-Tsushima Island in mid-1980s. At present, Taishu horses living outside of Tsushima Island, hereafter referred to as Non-Tsushima Taishus (NTTs), are tagged. However, the genetic structure of the NTT individuals remains unclear, and such individuals are not included in the current mating plans for Taishu horses. Herein, we examined the genetic structure of 18 NTT individuals by comparing their genomic (SNP) information with that of individuals on Tsushima Island (TT), four other native Japanese breeds, and one Anglo-Arabian breed by using ddRAD-seq. We found that all individuals related to the Taishu can be grouped in one cluster, which was separated from other horse breeds. Patterns of specific and shared SNPs in NTT individuals closely resembled those of TT individuals, suggesting very minor genetic differences. Meanwhile, the heterozygosity of NTT individuals was slightly higher than that of TT individuals, and many NTT individuals were of fertile age, suggesting that the pedigree of NTT individuals would be useful in breed conservation plans for Taishu horses. Based on their genomic information, we also reconstructed the pedigree structures of four NTT individuals with no family information. The inclusion of NTT individuals in future mating plans on Tsushima Island may be an effective and feasible method for conserving the Taishu horse breed in Japan.

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对马岛内外太舒马的遗传分析及其保护意义。
太马是日本本土品种,2018年在对马岛只剩下41匹。它们的遗传多样性被认为低于其他日本本土马品种;因此,需要对该品种的可持续保护进行研究。历史记录显示,在20世纪80年代中期,有几只太鱼被放生到对马岛附近。目前,对生活在对马岛以外的太马(以下简称非对马岛太马)进行了标记。然而,NTT个体的遗传结构仍不清楚,这些个体不包括在目前的太树马交配计划中。本文采用ddRAD-seq方法,将18个NTT个体的基因组(SNP)信息与对马岛(TT)、其他4个日本本土品种和1个英国-阿拉伯品种的基因组(SNP)信息进行比较,分析了NTT个体的遗传结构。结果表明,所有与太树有关的个体都可以归为一个群,与其他马品种分离。NTT个体的特异性和共享snp模式与TT个体非常相似,表明遗传差异非常小。同时,NTT个体的杂合度略高于TT个体,且许多NTT个体处于可育年龄,提示NTT个体的系谱可用于太树马的品种保护计划。基于基因组信息,我们还重建了4个没有家族信息的NTT个体的家系结构。将NTT个体纳入对马岛未来的交配计划可能是保护日本太马品种的有效可行方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Equine Science
Journal of Equine Science Veterinary-Equine
CiteScore
1.60
自引率
0.00%
发文量
9
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