BECN1 promotes the migration of NSCLC cells through regulating the ubiquitination of Vimentin.

IF 3.3 3区 生物学 Q3 CELL BIOLOGY
Zhujun Cheng, Hongbo Xin, Tianyu Han
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引用次数: 15

Abstract

BECN1/Beclin1 is one of the key proteins in autophagy regulation. However, the biological functions of BECN1 in non-small cell lung cancer (NSCLC) were obscure. Here, we found that neither BECN1 knockdown nor overexpression affected the proliferation of NSCLC cells. Surprisingly, BECN1 overexpression increased cell migration and knocking down BECN1 significantly reduced the migratory ability of NSCLC cells. We further demonstrated that BECN1 could interact with Vimentin and affected its K48-linked ubiquitination. What's more, BECN1 could also interact with ubiquitin-specific peptidase 14 (USP14), the key de-ubiquitinase of Vimentin, and regulated USP14 mediated de-ubiquitination of Vimentin. Thus, our studies revealed an oncosupportive role of BECN1 in the migration of NSCLC cells through regulating the ubiquitination of Vimentin.

Abstract Image

Abstract Image

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BECN1通过调控Vimentin泛素化促进NSCLC细胞的迁移。
BECN1/Beclin1是自噬调控的关键蛋白之一。然而BECN1在非小细胞肺癌(NSCLC)中的生物学功能尚不清楚。在这里,我们发现BECN1敲低和过表达都不影响NSCLC细胞的增殖。令人惊讶的是,BECN1过表达增加了细胞迁移,而敲低BECN1可显著降低NSCLC细胞的迁移能力。我们进一步证明BECN1可以与Vimentin相互作用并影响其k48连接的泛素化。BECN1还能与Vimentin的关键去泛素酶USP14相互作用,调控USP14介导的Vimentin去泛素化。因此,我们的研究揭示了BECN1通过调节Vimentin泛素化在NSCLC细胞迁移中的促癌作用。
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来源期刊
CiteScore
6.40
自引率
0.00%
发文量
7
审稿时长
53 weeks
期刊介绍: Cell Adhesion & Migration is a multi-disciplinary, peer reviewed open access journal that focuses on the biological or pathological implications of cell-cell and cell-microenvironment interactions. The main focus of this journal is fundamental science. The journal strives to serve a broad readership by regularly publishing review articles covering specific disciplines within the field, and by publishing focused issues that provide an overview on specific topics of interest within the field. Cell Adhesion & Migration publishes relevant and timely original research, as well as authoritative overviews, commentaries, and perspectives, providing context for the work presented in Cell Adhesion & Migration and for key results published elsewhere. Original research papers may cover all topics important in the field of cell-cell and cell-matrix interactions. Cell Adhesion & Migration also publishes articles related to cell biomechanics, biomaterial, and development of related imaging technologies.
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