{"title":"Visualization and Quantification of Digitally Dissected Muscle Fascicles in the Masticatory Muscles of Callithrix jacchus Using Nondestructive DiceCT.","authors":"Edwin Dickinson, Colin Basham, Avadh Rana, Adam Hartstone-Rose","doi":"10.1002/ar.24212","DOIUrl":null,"url":null,"abstract":"<p><p>The organization and length of a muscle's fascicles imparts its contractile properties. Longer fascicles permit increased muscle excursion, whereas changes in fascicle orientation relate to the overall vector of contractile force. Collecting data on fascicle architecture has traditionally involved destructive and irreversible gross dissection. In recent years, however, new imaging modalities have permitted muscles and their fascicles to be visualized nondestructively. Here, we present data from a primate (Callithrix jacchus), in which, for the first time, individual muscle fascicles are digitally \"dissected\" (segmented and reconstructed) using nondestructive, high-resolution diffusible iodine-based contrast-enhanced computed tomography (DiceCT) techniques. We also present quantitative data on the length and orientation of these fascicles within 10 muscle divisions of the jaw adductor and abductor musculature (superficial, deep, and zygomatic portions of temporalis and masseter; medial and lateral pterygoid; anterior and posterior digastric) and compare these digitally measured lengths to fascicular lengths measured using traditional gross and chemical dissection. Digitally derived fascicle lengths correspond well to their dissection-derived counterparts. Moreover, our analyses of changes in fascicle orientation across the adductor complex enable us to visualize previously uncharacterized levels of detail and highlight significant variation between adjacent muscle layers within muscle groups (e.g., between superficial, deep, and zygomatic portions of masseter and temporalis). We conclude that this technique offers great potential to future research, particularly for questions centered around the visualization and quantification of obscured and often-overlooked muscles such as the pterygoid and digastric muscles, and for deriving more accurate models of the masticatory system as a whole. Anat Rec, 302:1891-1900, 2019. © 2019 American Association for Anatomy.</p>","PeriodicalId":520555,"journal":{"name":"Anatomical record (Hoboken, N.J. : 2007)","volume":" ","pages":"1891-1900"},"PeriodicalIF":2.1000,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/ar.24212","citationCount":"14","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Anatomical record (Hoboken, N.J. : 2007)","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1002/ar.24212","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2019/7/12 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 14
Abstract
The organization and length of a muscle's fascicles imparts its contractile properties. Longer fascicles permit increased muscle excursion, whereas changes in fascicle orientation relate to the overall vector of contractile force. Collecting data on fascicle architecture has traditionally involved destructive and irreversible gross dissection. In recent years, however, new imaging modalities have permitted muscles and their fascicles to be visualized nondestructively. Here, we present data from a primate (Callithrix jacchus), in which, for the first time, individual muscle fascicles are digitally "dissected" (segmented and reconstructed) using nondestructive, high-resolution diffusible iodine-based contrast-enhanced computed tomography (DiceCT) techniques. We also present quantitative data on the length and orientation of these fascicles within 10 muscle divisions of the jaw adductor and abductor musculature (superficial, deep, and zygomatic portions of temporalis and masseter; medial and lateral pterygoid; anterior and posterior digastric) and compare these digitally measured lengths to fascicular lengths measured using traditional gross and chemical dissection. Digitally derived fascicle lengths correspond well to their dissection-derived counterparts. Moreover, our analyses of changes in fascicle orientation across the adductor complex enable us to visualize previously uncharacterized levels of detail and highlight significant variation between adjacent muscle layers within muscle groups (e.g., between superficial, deep, and zygomatic portions of masseter and temporalis). We conclude that this technique offers great potential to future research, particularly for questions centered around the visualization and quantification of obscured and often-overlooked muscles such as the pterygoid and digastric muscles, and for deriving more accurate models of the masticatory system as a whole. Anat Rec, 302:1891-1900, 2019. © 2019 American Association for Anatomy.
应用无损成像技术对棘鱼咀嚼肌数字解剖肌束的可视化和定量分析。
肌肉束的组织和长度决定了它的收缩特性。较长的肌束允许增加肌肉偏移,而肌束方向的变化与收缩力的总矢量有关。收集束状结构的数据传统上涉及破坏性和不可逆的大体解剖。然而,近年来,新的成像方式已经允许非破坏性地可视化肌肉及其肌束。在这里,我们展示了灵长类动物(Callithrix jacchus)的数据,其中首次使用无损的、高分辨率的扩散碘基对比增强计算机断层扫描(DiceCT)技术对单个肌肉束进行了数字“解剖”(分割和重建)。我们还提供了在下颌内收肌和外展肌的10个肌区(颞肌和咬肌的浅部、深部和颧部)内这些肌束的长度和方向的定量数据;内外侧翼状肌;前后二腹肌),并将这些数字测量的长度与传统的大体和化学解剖测量的束状肌长度进行比较。数字衍生的束长度与解剖衍生的束长度很好地对应。此外,我们对内收肌复合体的肌束方向变化的分析使我们能够可视化以前未表征的细节水平,并突出肌肉群内邻近肌肉层之间的显著差异(例如,咬肌和颞肌的浅表、深层和颧部之间)。我们的结论是,这项技术为未来的研究提供了巨大的潜力,特别是围绕模糊和经常被忽视的肌肉(如翼状肌和二腹肌)的可视化和量化问题,以及推导更准确的咀嚼系统整体模型。生物医学工程学报,32(2):391 - 391,2019。©2019美国解剖学协会。
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