Concentrate Growth Factors Regulate Osteogenic Dysfunction of MC3T3-E1 Cells Induced by High Glucose Through PI3K/Akt Signaling Pathway.

3区医学 Q1 Dentistry

Implant Dentistry Pub Date : 2019-10-01 DOI:10.1097/ID.0000000000000921

Kai Dong, Pengjie Hao, Wenjuan Zhou, Zhonghao Liu

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引用次数: 4

Abstract

Introduction: The aim of this study is to investigate the effects of Concentrate Growth Factors Extract (CGF-e) on the proliferation and osteogenic differentiation of MC3T3-E1 cells under high glucose condition.

Materials and methods: MC3T3-E1 cells were divided into 4 groups including normal glucose (5.5-mM) group (control), high glucose (25.5-mM) group, normal glucose + CGF-e group, and high glucose + CGF-e group. The proliferation, osteogenic differentiation and mineralization of osteoblasts were evaluated, respectively, by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, cytoskeleton analysis, alkaline phosphatase activity assay, alizarin red staining, and real-time polymerase chain reaction. Western blots analysis was used to explore the role of PI3K/Akt pathway.

Results: The viability, osteogenic differentiation, and mineralization of MC3T3-E1 cells were significantly decreased by high glucose. All observed osteogenic dysfunction were inhibited by CGF-e. Moreover, the PI3K/Akt pathway was activated by CGF-e.

Conclusions: It was concluded that the soluble factors released by CGF could significantly attenuate high glucose-mediated MC3T3-E1 cells osteogenic dysfunction through the PI3K/Akt pathway.

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浓缩生长因子通过PI3K/Akt信号通路调控高糖诱导的MC3T3-E1细胞成骨功能障碍

前言:本研究旨在探讨浓缩生长因子提取物(CGF-e)对高糖条件下MC3T3-E1细胞增殖和成骨分化的影响。材料与方法:将MC3T3-E1细胞分为正常葡萄糖(5.5 mm)组(对照)、高糖(25.5 mm)组、正常葡萄糖+ CGF-e组、高糖+ CGF-e组4组。采用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑(MTT)法、细胞骨架法、碱性磷酸酶活性法、茜素红染色法和实时聚合酶链反应法分别评价成骨细胞的增殖、成骨分化和矿化情况。Western blots分析PI3K/Akt通路的作用。结果:高糖显著降低MC3T3-E1细胞活力、成骨分化和矿化。所有观察到的成骨功能障碍均被CGF-e抑制。此外，CGF-e可激活PI3K/Akt通路。结论:CGF释放的可溶性因子可通过PI3K/Akt通路显著减弱高糖介导的MC3T3-E1细胞成骨功能障碍。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Implant Dentistry 医学-牙科与口腔外科

CiteScore

4.00

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Cessation. Implant Dentistry, an interdisciplinary forum for general practitioners, specialists, educators, and researchers, publishes relevant clinical, educational, and research articles that document current concepts of oral implantology in sections on biomaterials, clinical reports, oral and maxillofacial surgery, oral pathology, periodontics, prosthodontics, and research. The journal includes guest editorials, letters to the editor, book reviews, abstracts of current literature, and news of sponsoring societies.