Identification of Quinolone and Colistin Resistance Genes in Escherichia Coli Strains Isolated from Mucosal Samples of Patients with Colorectal Cancer and Healthy Subjects.

Q3 Medicine
Hassan Mahmoudi, Sima Ghiasvand, Omid Zarei, Hadi Hossainpour, Mohammad Y Alikhani
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引用次数: 2

Abstract

Introduction: Antibiotic resistance and extensive use of antibiotics are amongst the major causes of failure in antibiotic treatment. The purpose of this study was to investigate antibiotic resistance patterns and to identify resistance genes of quinolones and colistin in Escherichia coli. There are a very few patents on E. coli isolated from colorectal cancer. So, this study demonstrates that some bacteria resistant to ciprofloxacin have not resistance genes.Moreover, new patterns for E. coli are presented for isolates of patients with colorectal cancer.

Materials and methods: Of the three healthy people, inflammatory bowel diseases (IBD) patients and colorectal cancer patients, 40 E. coli strains isolated after confirmation by biochemical and molecular methods. The susceptibility of isolates to antibiotics was investigated using disk diffusion test. After deoxyribonucleic acid (DNA) extraction, polymerase chain reaction (PCR) was used to identify genes encoding resistance to ciprofloxacin (qnr A, qnr B) and colistin (mcr-1).

Results: The results showed that E. coli isolates from colorectal cancer patients had the highest resistance to piperacillin (67.5%), ceftazidime (47.5%), and cefepime (42.5%). Also, E. coli strains isolated from IBD patients showed resistance to antibiotic ceftazidime 13%. More than 95% of E. coli strains isolated from healthy people were susceptible to antibiotics. Based on the results, 18 (15%) E. coli strains showed resistance to ciprofloxacin. The qnr A gene was detected in 61.11% isolates; however, qnr B was detected in 9 (50%) isolates. Isolates resistant to colistin were not observed.

Conclusion: These findings indicate increased resistance of E. coli to ciprofloxacin in comparison with prior studies. Further research in this field will increase our knowledge and more effective exposure to the antibiotic resistance of the pathogenic microorganisms.

Abstract Image

Abstract Image

结直肠癌患者及健康人粘膜标本中大肠杆菌耐喹诺酮和粘菌素基因的鉴定
抗生素耐药性和广泛使用抗生素是抗生素治疗失败的主要原因之一。本研究的目的是调查抗生素耐药模式,并确定喹诺酮类和粘菌素在大肠杆菌中的耐药基因。从结直肠癌中分离出的大肠杆菌很少有专利。因此,这项研究表明,一些对环丙沙星耐药的细菌没有耐药基因。此外,大肠杆菌的新模式提出了分离结肠直肠癌患者。材料与方法:从3名健康人群、炎症性肠病(IBD)患者和结直肠癌患者中分离出40株大肠杆菌,经生化和分子方法证实。采用纸片扩散试验研究分离菌株对抗生素的敏感性。提取脱氧核糖核酸(DNA)后,采用聚合酶链反应(PCR)鉴定对环丙沙星(qnr A, qnr B)和粘菌素(mcr-1)的耐药基因。结果:大肠杆菌对哌拉西林(67.5%)、头孢他啶(47.5%)和头孢吡肟(42.5%)的耐药率最高。此外,从IBD患者中分离的大肠杆菌菌株对抗生素头孢他啶的耐药性为13%。从健康人群中分离出的大肠杆菌菌株中,95%以上对抗生素敏感。结果显示,18株(15%)大肠杆菌对环丙沙星耐药。61.11%分离株中检出qnr A基因;9株(50%)分离株检出qnr B。未观察到对粘菌素耐药的分离株。结论:这些发现表明,与先前的研究相比,大肠杆菌对环丙沙星的耐药性增加。在这一领域的进一步研究将增加我们的知识和更有效地暴露于病原微生物的抗生素耐药性。
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来源期刊
Recent patents on anti-infective drug discovery
Recent patents on anti-infective drug discovery Medicine-Pharmacology (medical)
CiteScore
2.40
自引率
0.00%
发文量
1
期刊介绍: Recent Patents on Anti-Infective Drug Discovery publishes review articles on recent patents in the field of anti-infective drug discovery e.g. novel bioactive compounds, analogs & targets. A selection of important and recent patents on anti-infective drug discovery is also included in the journal. The journal is essential reading for all researchers involved in anti-infective drug design and discovery.
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