Identification of MazF Homologue in Legionella pneumophila Which Cleaves RNA at the AACU Sequence.

IF 1.2 Q2 Biochemistry, Genetics and Molecular Biology

Journal of Molecular Microbiology and Biotechnology Pub Date : 2018-01-01 Epub Date: 2019-03-20 DOI:10.1159/000497146

Mao Shaku, Jung-Ho Park, Masayori Inouye, Yoshihiro Yamaguchi

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引用次数: 4

Abstract

MazF is a sequence-specific endoribonuclease that is widely conserved in bacteria and archaea. Here, we found an MazF homologue (MazF-lp; LPO-p0114) in Legionella pneumophila. The mazF-lp gene overlaps 14 base pairs with the upstream gene mazE-lp (MazE-lp; LPO-p0115). The induction of mazF-lp caused cell growth arrest, while mazE-lp co-induction recovered cell growth in Escherichia coli. In vivo and in vitro primer extension experiments showed that MazF-lp is a sequence-specific endoribonuclease cleaving RNA at AACU. The endoribonuclease activity of purified MazF-lp was inhibited by purified MazE-lp. We found that MazE-lp and the MazEF-lp complex specifically bind to the palindromic sequence present in the 5'-untranslated region of the mazEF-lp operon. MazE-lp and MazEF-lp both likely function as a repressor for the mazEF-lp operon and for other genes, including icmR, whose gene product functions as a secretion chaperone for the IcmQ pore-forming protein, by specifically binding to the palindromic sequence in 5'-UTR of these genes.

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在AACU序列上切割RNA的嗜肺军团菌MazF同源物的鉴定。

MazF是一种序列特异性核糖核酸内切酶，在细菌和古细菌中广泛保守。在这里，我们发现了一个MazF同源物(MazF-lp;嗜肺军团菌LPO-p0114)。mazF-lp基因与上游基因mazE-lp (mazE-lp;LPO-p0115)。在大肠杆菌中，mazF-lp的诱导导致细胞生长停滞，而mazE-lp的共诱导使细胞恢复生长。体内和体外引物延伸实验表明，MazF-lp是AACU上序列特异性核糖核酸内切酶切割RNA。纯化后的MazF-lp核糖核酸内酶活性被纯化后的MazE-lp抑制。我们发现，MazE-lp和MazE-lp复合物特异性结合在MazE-lp操纵子的5'-未翻译区域的回文序列上。MazE-lp和MazEF-lp都可能作为MazEF-lp操纵子和其他基因的抑制因子，包括icmR，其基因产物通过特异性结合这些基因的5'-UTR中的回语序列，作为IcmQ孔形成蛋白的分泌伴侣。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Molecular Microbiology and Biotechnology 生物-生物工程与应用微生物

CiteScore

3.90

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： We are entering a new and exciting era of microbiological study and application. Recent advances in the now established disciplines of genomics, proteomics and bioinformatics, together with extensive cooperation between academic and industrial concerns have brought about an integration of basic and applied microbiology as never before.