T lymphocytes migrate upstream after completing the leukocyte adhesion cascade.

IF 3.3 3区 生物学 Q3 CELL BIOLOGY
Cell Adhesion & Migration Pub Date : 2019-12-01 Epub Date: 2019-03-17 DOI:10.1080/19336918.2019.1587269
Nicholas R Anderson, Alexander Buffone, Daniel A Hammer
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引用次数: 17

Abstract

The leukocyte adhesion cascade is of critical importance for both the maintenance of immune homeostasis and the ability of immune cells to perform effector functions. Here, we present data showing CD4+ T cells migrate upstream (against the direction of flow) after completing the leukocyte adhesion cascade on surfaces displaying either ICAM-1 or ICAM-1 and VCAM-1, but migrate downstream on surfaces displaying only VCAM-1. Cells completing the cascade on HUVECs initially migrate upstream before reverting to more random migration, partly caused by transmigration of cells migrating against the flow. Furthermore, cells migrating upstream transmigrate faster than cells migrating downstream. On HUVECs, blocking interactions between LFA-1 and ICAM-1 resulted in downstream migration and slower transmigration. These results further suggest a possible physiological role for upstream migration in vivo.

Abstract Image

Abstract Image

T淋巴细胞完成白细胞粘附级联后向上游迁移。
白细胞粘附级联对于维持免疫稳态和免疫细胞执行效应功能的能力至关重要。在这里,我们提供的数据显示,CD4+ T细胞在完成白细胞粘附级联后,在显示ICAM-1或ICAM-1和VCAM-1的表面上向上游(逆流动方向)迁移,但在仅显示VCAM-1的表面上向下游迁移。在HUVECs上完成级联的细胞最初向上游迁移,然后恢复到更随机的迁移,部分原因是细胞逆流迁移。此外,上游迁移的细胞比下游迁移的细胞迁移得更快。在HUVECs上,阻断LFA-1和ICAM-1之间的相互作用导致下游迁移和较慢的迁移。这些结果进一步表明,在体内上游迁移可能具有生理作用。
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来源期刊
CiteScore
6.40
自引率
0.00%
发文量
7
审稿时长
53 weeks
期刊介绍: Cell Adhesion & Migration is a multi-disciplinary, peer reviewed open access journal that focuses on the biological or pathological implications of cell-cell and cell-microenvironment interactions. The main focus of this journal is fundamental science. The journal strives to serve a broad readership by regularly publishing review articles covering specific disciplines within the field, and by publishing focused issues that provide an overview on specific topics of interest within the field. Cell Adhesion & Migration publishes relevant and timely original research, as well as authoritative overviews, commentaries, and perspectives, providing context for the work presented in Cell Adhesion & Migration and for key results published elsewhere. Original research papers may cover all topics important in the field of cell-cell and cell-matrix interactions. Cell Adhesion & Migration also publishes articles related to cell biomechanics, biomaterial, and development of related imaging technologies.
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