Molecular characterization and expression of α-gliadin genes from wheat cultivar Dacke in Bg 250 rice variety.

IF 4.5 2区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Pub Date : 2019-01-01 Epub Date: 2019-05-29 DOI:10.1080/21645698.2019.1622990

Kirushanthy Kajendran, Naduviladath Vishvanath Chandrasekharan, Chamari Madhu Hettiarachchi, Wijerupage Sandhya Sulochana Wijesundera

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引用次数: 3

Abstract

The main seed storage protein in wheat is gluten. It consists of gliadin and glutenins. Gluten gives high elasticity and extensibility during bread making, facilitating the formation of the dough. Rice is the staple food of Sri Lankans but, it has poor dough making ability compared to wheat. The aim of the present work was to characterize, clone and express α-gliadin in the T₀ generation of Bg 250 rice variety as a preliminary step in improving the dough making ability of rice flour. Five α-gliadin recombinant pCR™2.1-TOPO® clones were selected for sequence analysis. Of the five clones, two functional genes and three pseudogenes were identified. Phylogenetic analysis revealed the two functional genes, (accession numbers KC660359 and KC660358) to be closely related to the α-gliadin genes of Triticum monococcum. The α-gliadin gene (KC660359) contained five cysteine residues, one less than the normal occurrence of cysteine residues in α-gliadin genes. To date there are no reports on expression of gliadin gene in transgenic rice. This novel gene was successfully expressed in the Sri Lankan rice variety Bg 250 under the control of the rice GluB-1 endosperm specific promoter.

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小麦品种Dacke的α-醇溶蛋白基因在Bg250水稻中的分子特征及表达。

小麦中主要的种子贮藏蛋白是谷蛋白。它由醇溶蛋白和臀大蛋白组成。面筋在面包制作过程中具有很高的弹性和延展性，有利于面团的形成。大米是斯里兰卡人的主食，但与小麦相比，它的面团制作能力较差。本工作的目的是在Bg250水稻品种T0代中鉴定、克隆和表达α-醇溶蛋白，作为提高米粉面团制作能力的初步步骤。五种α-醇溶蛋白重组pCR™选择2.1-TOPO®克隆进行序列分析。在五个克隆中，鉴定出两个功能基因和三个假基因。系统发育分析表明，这两个功能基因（登录号KC660359和KC660358）与单球菌小麦的α-醇溶蛋白基因密切相关。α-醇溶蛋白基因（KC660359）含有5个半胱氨酸残基，比正常α-醇蛋白基因中半胱氨酸残基少1个。到目前为止，还没有关于醇溶蛋白基因在转基因水稻中表达的报道。在水稻GluB-1胚乳特异性启动子的控制下，该新基因在斯里兰卡水稻品种Bg250中成功表达。

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来源期刊

Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

8.10

自引率

10.30%

发文量

期刊介绍： GM Crops & Food - Biotechnology in Agriculture and the Food Chain aims to publish high quality research papers, reviews, and commentaries on a wide range of topics involving genetically modified (GM) crops in agriculture and genetically modified food. The journal provides a platform for research papers addressing fundamental questions in the development, testing, and application of transgenic crops. The journal further covers topics relating to socio-economic issues, commercialization, trade and societal issues. GM Crops & Food aims to provide an international forum on all issues related to GM crops, especially toward meaningful communication between scientists and policy-makers. GM Crops & Food will publish relevant and high-impact original research with a special focus on novelty-driven studies with the potential for application. The journal also publishes authoritative review articles on current research and policy initiatives, and commentary on broad perspectives regarding genetically modified crops. The journal serves a wide readership including scientists, breeders, and policy-makers, as well as a wider community of readers (educators, policy makers, scholars, science writers and students) interested in agriculture, medicine, biotechnology, investment, and technology transfer. Topics covered include, but are not limited to: • Production and analysis of transgenic crops • Gene insertion studies • Gene silencing • Factors affecting gene expression • Post-translational analysis • Molecular farming • Field trial analysis • Commercialization of modified crops • Safety and regulatory affairs BIOLOGICAL SCIENCE AND TECHNOLOGY • Biofuels • Data from field trials • Development of transformation technology • Elimination of pollutants (Bioremediation) • Gene silencing mechanisms • Genome Editing • Herbicide resistance • Molecular farming • Pest resistance • Plant reproduction (e.g., male sterility, hybrid breeding, apomixis) • Plants with altered composition • Tolerance to abiotic stress • Transgenesis in agriculture • Biofortification and nutrients improvement • Genomic, proteomic and bioinformatics methods used for developing GM cops ECONOMIC, POLITICAL AND SOCIAL ISSUES • Commercialization • Consumer attitudes • International bodies • National and local government policies • Public perception, intellectual property, education, (bio)ethical issues • Regulation, environmental impact and containment • Socio-economic impact • Food safety and security • Risk assessments