Buffalo species identification and delineation using genetic barcoding markers

IF 3.5 Q3 Biochemistry, Genetics and Molecular Biology

Journal of Genetic Engineering and Biotechnology Pub Date : 2018-12-01 DOI:10.1016/j.jgeb.2018.07.006

Amal Ahmed Mohamed Hassan , Esraa Aly Balabel , Hanaa Abdel Sadek Oraby , Samy Anwar Darwish

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引用次数: 4

Abstract

Enrichment of barcode databases with mitochondrial cytochrome c oxidase subunit I (COI) barcode sequences in different animal taxa has become important for identification of animal source in food samples to prevent commercial fraud. In this study, COI barcode sequence in seventy one river buffalo samples were determined, analyzed and deposited in Genbank barcode database and barcode of life database (BOLD) to contribute for construction of public reference library for COI barcode sequence in river buffalo. Moreover COI barcode sequence was used to identify the closely related buffalo groups: river buffalo, swamp buffalo, lowland anoa and African buffalo. Results indicated the success of the COI barcode in the identification of each of the tested groups. Whereas a suggested sequence of other mitochondrial segment representing two successive transfer RNA (tRNA) genes; tRNA-Threonine (MT-TT) and tRNA-Proline (MT-TP) was failed to be used as a barcode marker for differentiation between the tested buffalo groups.

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利用遗传条形码标记进行水牛物种鉴定和圈定

利用线粒体细胞色素c氧化酶亚基I (COI)条形码序列丰富不同动物类群的条形码数据库，对于食品样品中动物来源的识别和防止商业欺诈具有重要意义。本研究对71份河水牛样本的COI条形码序列进行了测定、分析，并将其存入Genbank条形码数据库和生命条形码数据库(BOLD)，为建设河水牛COI条形码序列公共参考库做出贡献。利用COI条形码序列对近缘水牛类群(河水牛、沼泽水牛、低地水牛和非洲水牛)进行了鉴定。结果表明，COI条形码成功地识别了每个测试组。鉴于其他线粒体片段的建议序列代表两个连续的转移RNA (tRNA)基因;trna -苏氨酸(MT-TT)和trna -脯氨酸(MT-TP)不能作为区分水牛群体的条形码标记。

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来源期刊

Journal of Genetic Engineering and Biotechnology Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

5.70

自引率

5.70%

发文量

159

审稿时长

16 weeks

期刊介绍： Journal of genetic engineering and biotechnology is devoted to rapid publication of full-length research papers that leads to significant contribution in advancing knowledge in genetic engineering and biotechnology and provide novel perspectives in this research area. JGEB includes all major themes related to genetic engineering and recombinant DNA. The area of interest of JGEB includes but not restricted to: •Plant genetics •Animal genetics •Bacterial enzymes •Agricultural Biotechnology, •Biochemistry, •Biophysics, •Bioinformatics, •Environmental Biotechnology, •Industrial Biotechnology, •Microbial biotechnology, •Medical Biotechnology, •Bioenergy, Biosafety, •Biosecurity, •Bioethics, •GMOS, •Genomic, •Proteomic JGEB accepts