Molecular diversity of internal transcribed spacer among the monoconidial isolates of Magnaporthe oryzae isolated from rice in Southern Karnataka, India

IF 3.5 Q3 Biochemistry, Genetics and Molecular Biology

Journal of Genetic Engineering and Biotechnology Pub Date : 2018-12-01 DOI:10.1016/j.jgeb.2018.05.008

D. Jagadeesh , M.K. Prasanna Kumar , R. Chandrakanth , N.S. Devaki

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引用次数: 15

Abstract

Blast disease of rice plant is caused by Magnaporthe oryzae (anamorph Pyricularia oryzae). This disease is recognized to be one of the most serious diseases of rice crop around the world. A total of 72 monoconidial isolates of M. oryzae obtained from blast disease samples collected around Southern Karnataka were characterized using internal transcribed spacers of the ribosomal DNA sequences. These were analyzed by comparing with already deposited sequences in GenBank database. It helped in diagnosing the invasive pathogen in all locations. Variability of rDNA sequences was found to be highly polymorphic with 0.068962 nucleotide diversity showing 6 distinct clades. 33 haplotype groups were identified with haplotype diversity of 0.8881 and Tajima's neutrality test with a D value of −1.96827 with P < 0.05 showing the presence of variations among the sequences of pathogen isolates. The Tajima’s D value of less than one indicates the presence of a high number of rare alleles. Our study indicates that the pathogen might have undergone recent selection pressure because of the exposure to a large number of cultivars resulting in the evolution of rare alleles. This shows the importance of characterizing internal transcribed spacer (ITS) to know pathogen diversity and its fitness which has potential to contribute to the field of breeding for blast disease resistance.

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印度卡纳塔克邦南部水稻Magnaporthe oryzae单分生菌株内部转录间隔序列的分子多样性

水稻稻瘟病是由稻瘟病菌(稻瘟病菌)引起的。该病是世界上公认的危害水稻作物最严重的病害之一。利用核糖体DNA序列的内部转录间隔序列对从卡纳塔克邦南部爆炸病样本中获得的72株单分生分离株进行了鉴定。通过比较GenBank数据库中已存序列进行分析。它有助于诊断所有地区的侵袭性病原体。rDNA序列具有高度多态性，核苷酸多样性为0.068962，具有6个不同的进化支。共鉴定出33个单倍型群体，单倍型多样性为0.8881，田岛中性检验的D值为- 1.96827,P值 < 0.05，表明病原菌分离物序列之间存在差异。田岛的D值小于1表明存在大量的稀有等位基因。我们的研究表明，病原菌可能经历了最近的选择压力，因为暴露于大量的栽培品种，导致稀有等位基因的进化。这表明鉴定内部转录间隔序列(ITS)对了解病原菌多样性及其适应度的重要性，并有可能为稻瘟病抗性育种领域做出贡献。

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来源期刊

Journal of Genetic Engineering and Biotechnology Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

5.70

自引率

5.70%

发文量

159

审稿时长

16 weeks

期刊介绍： Journal of genetic engineering and biotechnology is devoted to rapid publication of full-length research papers that leads to significant contribution in advancing knowledge in genetic engineering and biotechnology and provide novel perspectives in this research area. JGEB includes all major themes related to genetic engineering and recombinant DNA. The area of interest of JGEB includes but not restricted to: •Plant genetics •Animal genetics •Bacterial enzymes •Agricultural Biotechnology, •Biochemistry, •Biophysics, •Bioinformatics, •Environmental Biotechnology, •Industrial Biotechnology, •Microbial biotechnology, •Medical Biotechnology, •Bioenergy, Biosafety, •Biosecurity, •Bioethics, •GMOS, •Genomic, •Proteomic JGEB accepts