Fluid shear stress induces cell migration and invasion via activating autophagy in HepG2 cells.

IF 3.3 3区 生物学 Q3 CELL BIOLOGY
Cell Adhesion & Migration Pub Date : 2019-12-01 Epub Date: 2019-01-31 DOI:10.1080/19336918.2019.1568141
Zhiping Yan, Guanyue Su, Wenbo Gao, Jia He, Yang Shen, Ye Zeng, Xiaoheng Liu
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引用次数: 25

Abstract

Fluid shear stress (FSS) regulates the metastasis of hepatocellular carcinoma (HCC). In the present study, we aimed to study the role of autophagy in HCC cells under FSS. The results showed that FSS upregulated the protein markers of autophagy, induced LC3B aggregation and formation of autophagosomes. Inhibition of integrin by Cliengitide (Cli) or inhibition of the microfilaments formation both inhibited the activation of autophagy in HepG2 under FSS. In addition, Cli inhibited the microfilaments formation and expressions of Rac1 and RhoA in HepG2 cells under FSS. Finally, inhibition of autophagy suppressed the cell migration and invasion in HepG2 under FSS. In conclusion, FSS induced autophagy to promote migration and invasion of HepG2 cells via integrin/cytoskeleton pathways.

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流体剪切应力通过激活HepG2细胞的自噬诱导细胞迁移和侵袭。
流体剪切应力(FSS)调控肝细胞癌(HCC)的转移。在本研究中,我们旨在研究FSS作用下自噬在HCC细胞中的作用。结果表明,FSS上调自噬蛋白标志物,诱导LC3B聚集,形成自噬小体。Cliengitide (Cli)抑制整合素或抑制微丝形成均可抑制FSS作用下HepG2细胞自噬的激活。此外,Cli抑制了FSS下HepG2细胞微丝的形成以及Rac1和RhoA的表达。最后,抑制自噬抑制了FSS作用下HepG2细胞的迁移和侵袭。综上所述,FSS诱导自噬通过整合素/细胞骨架通路促进HepG2细胞的迁移和侵袭。
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来源期刊
CiteScore
6.40
自引率
0.00%
发文量
7
审稿时长
53 weeks
期刊介绍: Cell Adhesion & Migration is a multi-disciplinary, peer reviewed open access journal that focuses on the biological or pathological implications of cell-cell and cell-microenvironment interactions. The main focus of this journal is fundamental science. The journal strives to serve a broad readership by regularly publishing review articles covering specific disciplines within the field, and by publishing focused issues that provide an overview on specific topics of interest within the field. Cell Adhesion & Migration publishes relevant and timely original research, as well as authoritative overviews, commentaries, and perspectives, providing context for the work presented in Cell Adhesion & Migration and for key results published elsewhere. Original research papers may cover all topics important in the field of cell-cell and cell-matrix interactions. Cell Adhesion & Migration also publishes articles related to cell biomechanics, biomaterial, and development of related imaging technologies.
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