[Evaluation of a Newly Developed Reagent "Lias Auto P-FDP" on Coapresta 2000 and Clinico-Pathological Analysis of Discrepant Samples in FDP Value].

Maki Kondo, Takako Tamura, Hitomi Miura, Asako Sato
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Abstract

Patients with disseminated intravascular coagulation (DIC) exhibit increased levels of fibrin/fibrinogen degradation products (FDP), and FDP levels are carefully monitored as a marker of fibrinolysis during the diagnosis of DIC and other fibrinolysis-related conditions. Although FDP levels can be measured using automatic analyzers, it is reported that measured FDP values differ depending on the reagent used. Recently, a newly developed reagent for measuring FDP levels (Lias Auto P-FDP) was launched. In this study, we used Lias Auto P-FDP in combination with the Coapresta 2000 automatic analyzer and evaluated its reactivity. We confirmed the reproducibility of the measurements obtained with the Lias Auto P-FDP reagent when the manufacturer's instructions were followed and that the Lias Auto P-FDP reagent can be used with the Coapresta 2000. In the reactivity test, the Lias Auto P-FDP reagent exhibited stronger reactivity with low molecular weight FDP than the reagent we currently use (BL2 P-FDP). Moreover, the samples that exhibited non-specific reactions to the BL2 P-FDP reagent did not display similar reactions to the Lias auto P-FDP reagent. In the clinico-pathological analysis of divergent value between Lias Auto P-FDP and BL2 P-FDP, seven cases were highly discrepant value of FDP. Interestingly, we found the description of pleural fluid and/or ascites in 85.7% cases. In conclusion, we confirmed that the Lias Auto P-FDP reagent can be used in combination with the Coapresta 2000. In addition, the reactivity of FDP varied depending on the reagent used. It is important to understand the characteristics of each FDP reagent and which automatic analyzers they can be used with. [Original].

新开发试剂Lias Auto P-FDP对Coapresta 2000的评价及FDP值差异样品的临床病理分析
弥散性血管内凝血(DIC)患者表现出纤维蛋白/纤维蛋白原降解产物(FDP)水平升高,在DIC和其他纤维蛋白溶解相关疾病的诊断中,FDP水平被仔细监测作为纤维蛋白溶解的标志物。虽然FDP水平可以测量使用自动分析仪,据报道,测量的FDP值不同取决于所用的试剂。最近,一种新开发的测定FDP水平的试剂(Lias Auto P-FDP)问世。在本研究中,我们使用Lias Auto P-FDP与Coapresta 2000自动分析仪结合,并评估其反应性。我们确认了Lias Auto P-FDP试剂在遵循制造商说明的情况下测量结果的重复性,并且Lias Auto P-FDP试剂可以与Coapresta 2000一起使用。在反应性测试中,Lias Auto P-FDP试剂对低分子量FDP的反应性比我们目前使用的试剂(BL2 P-FDP)更强。此外,对BL2 P-FDP试剂表现出非特异性反应的样品与Lias auto P-FDP试剂没有类似的反应。在Lias Auto P-FDP值与BL2 P-FDP值差异的临床病理分析中,7例FDP值存在高度差异。有趣的是,我们发现85.7%的病例有胸腔积液和/或腹水。综上所述,Lias Auto P-FDP试剂可与Coapresta 2000联合使用。此外,FDP的反应性随所用试剂的不同而不同。了解每种FDP试剂的特性以及它们可以与哪些自动分析仪一起使用是很重要的。(最初的)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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