A protocol for generating induced T cells by reprogramming B cells in vivo

IF 4 Q2 CELL & TISSUE ENGINEERING

Cell Regeneration Pub Date : 2018-09-01 DOI:10.1016/j.cr.2018.05.001

Qitong Weng , Fangxiao Hu , Mengyun Zhang , Yong Dong , Cui Lv , Ying Wang , Xiaofei Liu , Jinyong Wang

引用次数: 1

Abstract

Obtaining T cells by reprogramming is one of the major goals in regenerative medicine. Here, we describe a protocol for generating functional T cells from Hoxb5-expressing pro/pre-B cells in vivo. This protocol includes the construction of Hoxb5 recombinant plasmids, retroviral packaging, isolation and viral transduction of pro/pre-B cells, cell transplantation, and phenotypic analysis of induced T cells. The procedure is reproducible and straightforward, providing an approach for generating induced T cells for translational research.

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一种在体内通过对B细胞重编程产生诱导T细胞的方案

通过重编程获得T细胞是再生医学的主要目标之一。在这里，我们描述了一种在体内从表达hoxb5的pro/pre-B细胞中生成功能性T细胞的方案。该方案包括Hoxb5重组质粒的构建、逆转录病毒包装、pro/pre-B细胞的分离和病毒转导、细胞移植和诱导T细胞的表型分析。这个过程是可重复的和直接的，为产生用于翻译研究的诱导T细胞提供了一种方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell Regeneration Biochemistry, Genetics and Molecular Biology-Cell Biology

CiteScore

5.80

自引率

0.00%

发文量

审稿时长

35 days

期刊介绍： Cell Regeneration aims to provide a worldwide platform for researches on stem cells and regenerative biology to develop basic science and to foster its clinical translation in medicine. Cell Regeneration welcomes reports on novel discoveries, theories, methods, technologies, and products in the field of stem cells and regenerative research, the journal is interested, but not limited to the following topics: ◎ Embryonic stem cells ◎ Induced pluripotent stem cells ◎ Tissue-specific stem cells ◎ Tissue or organ regeneration ◎ Methodology ◎ Biomaterials and regeneration ◎ Clinical translation or application in medicine