Hyaluronan-CD44 interactions mediate contractility and migration in periodontal ligament cells.

IF 3.3 3区生物学 Q3 CELL BIOLOGY

Cell Adhesion & Migration Pub Date : 2019-12-01 Epub Date: 2019-02-08 DOI:10.1080/19336918.2019.1568140

Zeinab Al-Rekabi, Adriane M Fura, Ilsa Juhlin, Alaa Yassin, Tracy E Popowics, Nathan J Sniadecki

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引用次数: 12

Abstract

The role of hyaluronan (HA) in periodontal healing has been speculated via its interaction with the CD44 receptor. While HA-CD44 interactions have previously been implicated in numerous cell types; effect and mechanism of exogenous HA on periodontal ligament (PDL) cells is less clear. Herein, we examine the effect of exogenous HA on contractility and migration in human and murine PDL cells using arrays of microposts and time-lapse microscopy. Our findings observed HA-treated human PDL cells as more contractile and less migratory than untreated cells. Moreover, the effect of HA on contractility and focal adhesion area was abrogated when PDL cells were treated with Y27632, an inhibitor of rho-dependent kinase, but not when these cells were treated with ML-7, an inhibitor of myosin light chain kinase. Our results provide insight into the mechanobiology of PDL cells, which may contribute towards the development of therapeutic strategies for periodontal healing and tissue regeneration.

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透明质酸- cd44相互作用介导牙周韧带细胞的收缩和迁移。

透明质酸(HA)在牙周愈合中的作用已经通过其与CD44受体的相互作用进行了推测。虽然HA-CD44相互作用先前涉及许多细胞类型;外源性HA对牙周韧带(PDL)细胞的影响及其机制尚不清楚。在这里，我们使用微柱阵列和延时显微镜检查外源性HA对人和鼠PDL细胞收缩性和迁移的影响。我们的发现观察到ha处理的人PDL细胞比未处理的细胞更具收缩性和更少的迁移。此外，用rho依赖性激酶抑制剂Y27632处理PDL细胞时，HA对收缩性和局灶黏附面积的影响被消除，但用肌球蛋白轻链激酶抑制剂ML-7处理PDL细胞时，HA对收缩性和局灶黏附面积的影响没有被消除。我们的研究结果提供了对PDL细胞的机械生物学的深入了解，这可能有助于牙周愈合和组织再生的治疗策略的发展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell Adhesion & Migration CELL BIOLOGY-

CiteScore

6.40

自引率

0.00%

发文量

审稿时长

53 weeks

期刊介绍： Cell Adhesion & Migration is a multi-disciplinary, peer reviewed open access journal that focuses on the biological or pathological implications of cell-cell and cell-microenvironment interactions. The main focus of this journal is fundamental science. The journal strives to serve a broad readership by regularly publishing review articles covering specific disciplines within the field, and by publishing focused issues that provide an overview on specific topics of interest within the field. Cell Adhesion & Migration publishes relevant and timely original research, as well as authoritative overviews, commentaries, and perspectives, providing context for the work presented in Cell Adhesion & Migration and for key results published elsewhere. Original research papers may cover all topics important in the field of cell-cell and cell-matrix interactions. Cell Adhesion & Migration also publishes articles related to cell biomechanics, biomaterial, and development of related imaging technologies.