[Effect of Jianpi Shengxue Granule on Fertility and Early Embryo Development Toxicity of Rats].

Abstract

Objective To assess the effects of Jianpi Shengxue Granule (JSG) on the fertility and early embryo development in rats. Methods Totally 176 SD rats were stratified by sex, and divided into 4 groups, i.e., the control group, low, medium, high dose JSG treatment groups. Rats in the 3 JSG treatment groups were administered with JSG at 0, 1. 16, 3. 48, 5. 80 g/kg per day by gastrogavage, re- spectively. After 2 weeks of administration for females and 4 weeks of administration for males, males and females were caged in the ratio of 1:1. Females were administered to the gestation day 7. Males were administered for 59 -63 days until the day before anatomy. All parental generations were anatomized to observe signs and morphologies. Pathological examination was performed. General toxicity was detected. The testis and epididymis were weighed. Spermatozoa number was counted from epididymis, and repro- duction toxicity of sperm motility was checked. The numbers of corpus luteum, live fetus, dead fetus, and absorbed fetus were counted. The implantation number was calculated to observe early embryo de- velopment toxicity. Results (1) General toxicity: The body weight growth slowed down in male rats of the high dose JSG treatment group. No abnormality was found in low and medium dose JSG treatment groups. (2)Fertility toxicity: There was no obvious toxic effect on testis, epididymis, and spermatozoa number from epididymis sperm in all JSG treatment groups. (3) Early embryo development toxicity: No significant effect on the formation of early embryos was found in all JSG treatment groups. Conclusions Certain gastrointestinal toxicity of JSG might exist to some extent. The dose for non-adverse effect of JSG on fertility and early embryo development was 3. 38 g/kg per day. No obvious fertility or early embryo development toxicity occurred in each JSG treatment group.