[Effect of Water Extract of Ginseng on Biological Bechaviors of Lung Cancer A549 Cells and the Expression of F-actin in Co-culture System of TAMs and A549 Cells].

Abstract

Objective To study the effect of Water Extract of Ginseng (WEG) on the prolifera- tion/metastasis of lung cancer A549 cells and the expression of F-actin in co-culture system of tumor as- sociated macrophages (TAMs) and A549 cells. Methods Human acute leukemia mononuclear strain THP-1 was induced to become TAMs using Phorbol-12-myristate-13-acetate (PMA) combined IL-4 and IL- 13. The supernant of TAMs and A549 cells were co-cultured. A co-culture model was set up by simulating microenvironment of lung cancer. Then cells were divided into the blank control group (A549) , the co- culture group (A549 +TAMs) , high, middle, and low dose WEG groups (TAMs +A549 + high, middle, and low dose WEG). The effects of WEG on the proliferation/metastasis of lung cancer A549 cells and the expression of F-actin under various conditions were detected using MTT method, Real time cell analysis (RTCA) , and high content screening (HCS). Results Compared with the blank control group, the pro- liferation of A549 cells was obviously increased, cell migration was obviously elevated, and the area of cell skeleton was markedly enlarged in the TAMs + A549 group, with statistical difference (P <0. 05). Compared with the TAMs +A549 group, the proliferation and migration of A549 cells were inhibited, the area of cell skeleton and the number of microfilaments were reduced dose-dependently (P <0. 05). Conclusion WEG could effectively inhibit the proliferation and migration of A549 cells, which might be a- chieved by adjusting immunoactivities of TAMs, and further it affected biological behaviors of tumor cells.