Associations between environmental characteristics, high-resolution indoor microbiome, metabolome and allergic and non-allergic rhinitis symptoms for junior high school students†

IF 4.3 3区 环境科学与生态学 Q1 CHEMISTRY, ANALYTICAL
Xi Fu, Bingqian Du, Yi Meng, Yanling Li, Xunhua Zhu, Zheyuan Ou, Mei Zhang, Huarong Wen, Aminnuddin Ma'pol, Jamal Hisham Hashim, Zailina Hashim, Gunilla Wieslander, Qingmei Chen, Jun Jiang, Juan Wang, Dan Norbäck, Yun Xia, Qingsong Chen and Yu Sun
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引用次数: 1

Abstract

Rhinitis is one of the most prevalent chronic diseases globally. Microbiome exposure affects the occurrence of rhinitis. However, previous studies did not differentiate allergic rhinitis (AR) and non-allergic rhinitis (NAR) in the microbial association analysis. In this study, we investigate 347 students in 8 junior high schools, Terengganu, Malaysia, who were categorized as healthy (70.9%), AR (13.8%) and NAR (15.3%) based on a self-administered questionnaire and skin prick tests of pollen, pet, mould and house dust mite allergens. Classroom microbial and metabolite exposure in vacuumed dust was characterized by PacBio long-read amplicon sequencing, quantitative PCR and LC-MS-based untargeted metabolomics. Our findings indicate a similar microbial association pattern between AR and NAR. The richness in Gammaproteobacteria was negatively associated with AR and NAR symptoms, whereas total fungal richness was positively associated with AR and NAR symptoms (p < 0.05). Brasilonema bromeliae and Aeromonas enteropelogenes were negatively associated with AR and NAR, and Deinococcus was positively associated with AR and NAR (p < 0.01). Pipecolic acid was protectively associated with AR and NAR symptoms (OR = 0.06 and 0.13, p = 0.009 and 0.045). A neural network analysis showed that B. bromeliae was co-occurring with pipecolic acid, suggesting that the protective role of this species may be mediated by releasing pipecolic acid. Indoor relative humidity and the weight of vacuum dust were associated with AR and NAR, respectively (p < 0.05), but the health effects were mediated by two protective bacterial species, Aliinostoc morphoplasticum and Ilumatobacter fluminis. Overall, our study reported a similar microbial association pattern between AR and NAR and also revealed the complex interactions between microbial species, environmental characteristics, and rhinitis symptoms.

Abstract Image

环境特征、高分辨率室内微生物组、代谢组与初中生过敏性和非过敏性鼻炎症状的关系
鼻炎是全球最常见的慢性疾病之一。微生物群暴露会影响鼻炎的发生。然而,以往的研究并没有在微生物关联分析中区分变应性鼻炎(AR)和非变应性鼻炎(NAR)。本研究以马来西亚丁加奴市8所初中的347名学生为研究对象,通过问卷调查和花粉、宠物、霉菌和屋尘螨变应原皮肤点刺试验,分别为健康(70.9%)、AR(13.8%)和NAR(15.3%)。通过PacBio长读扩增子测序、定量PCR和基于lc - ms的非靶向代谢组学研究了教室微生物和代谢物在真空粉尘中的暴露。我们的研究结果表明AR和NAR之间存在类似的微生物关联模式。γ变形菌的丰富度与AR和NAR症状呈负相关,而真菌的总丰富度与AR和NAR症状呈正相关(p <0.05)。凤梨巴西单胞菌和肠产气单胞菌与AR和NAR呈负相关,球菌与AR和NAR呈正相关(p <0.01)。果酸与AR和NAR症状具有保护作用(OR = 0.06和0.13,p = 0.009和0.045)。神经网络分析表明,凤梨双歧杆菌与细果酸共存,表明该物种的保护作用可能是通过释放细果酸介导的。室内相对湿度和真空粉尘质量分别与AR和NAR相关(p <0.05),但两种保护性细菌Aliinostoc morphoplasticum和luminatobacter fluuminis介导了健康效应。总的来说,我们的研究报告了AR和NAR之间类似的微生物关联模式,也揭示了微生物种类、环境特征和鼻炎症状之间复杂的相互作用。
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来源期刊
Environmental Science: Processes & Impacts
Environmental Science: Processes & Impacts CHEMISTRY, ANALYTICAL-ENVIRONMENTAL SCIENCES
CiteScore
9.50
自引率
3.60%
发文量
202
审稿时长
1 months
期刊介绍: Environmental Science: Processes & Impacts publishes high quality papers in all areas of the environmental chemical sciences, including chemistry of the air, water, soil and sediment. We welcome studies on the environmental fate and effects of anthropogenic and naturally occurring contaminants, both chemical and microbiological, as well as related natural element cycling processes.
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