Tumor Pre-Analytics in Molecular Pathology: Impact on Protein Expression and Analysis.

Q1 Medicine

Current Pathobiology Reports Pub Date : 2018-01-01 Epub Date: 2018-09-06 DOI:10.1007/s40139-018-0179-5

Veronique M Neumeister, Hartmut Juhl

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引用次数: 16

Abstract

Purpose of review: Precision medicine promises patient tailored, individualized diagnosis and treatment of diseases and relies on clinical specimen integrity and accuracy of companion diagnostic testing. Therefore, pre-analytics, which are defined as the collection, processing, and storage of clinical specimens, are critically important to enable optimal diagnostics, molecular profiling, and clinical decision-making around harvested specimens. This review article discusses the impact of tumor pre-analytics on molecular pathology focusing on biospecimen protein expression and analysis.

Recent findings: Due to busy clinical schedules and workflows that have been established for many years and to lack of standardization and limited assessment tools to quantify variability in pre-analytical processing, the effects of pre-analytics on biospecimen integrity are often overlooked. Several studies have recently emphasized an emerging crisis in science and reproducibility of results.

Summary: Biomarker instability due to pre-analytical variables affects comprehensive analysis and molecular phenotyping of patients' tissue. This problematic emphasizes the critical need for standardized protocols and technologies to be applied in the clinical and research setting.

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分子病理学中的肿瘤前期分析:对蛋白质表达和分析的影响。

综述目的:精准医学承诺为患者量身定制、个体化的疾病诊断和治疗，依赖于临床标本的完整性和伴随诊断检测的准确性。因此，预分析，即临床标本的收集、处理和储存，对于实现最佳诊断、分子分析和临床决策至关重要。本文综述了肿瘤预分析对分子病理学的影响，重点是生物标本蛋白表达和分析。最近的发现:由于多年来建立的繁忙的临床时间表和工作流程，以及缺乏标准化和有限的评估工具来量化分析前处理的可变性，预分析对生物标本完整性的影响经常被忽视。最近有几项研究强调了科学和结果可重复性方面正在出现的危机。由于分析前变量导致的生物标志物不稳定性影响了患者组织的综合分析和分子表型。这一问题强调了在临床和研究环境中应用标准化方案和技术的迫切需要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Pathobiology Reports Medicine-Pathology and Forensic Medicine

CiteScore

6.40

自引率

0.00%

发文量

期刊介绍： This journal aims to offer expert review articles on the most important recent research pertaining to biological mechanisms underlying disease, including etiology, pathogenesis, and the clinical manifestations of cellular alteration. By providing clear, insightful, balanced contributions, the journal intends to serve those for whom the elucidation of new techniques and technologies related to pathobiology is essential. We accomplish this aim by appointing international authorities to serve as Section Editors in key subject areas across the field. Section Editors select topics for which leading experts contribute comprehensive review articles that emphasize new developments and recently published papers of major importance, highlighted by annotated reference lists. An Editorial Board of more than 20 internationally diverse members reviews the annual table of contents, ensures that topics include emerging research, and suggests topics of special importance to their country/region. Topics covered may include autophagy, cancer stem cells, induced pluripotential stem cells (iPS cells), inflammation and cancer, matrix pathobiology, miRNA in pathobiology, mitochondrial dysfunction/diseases, and myofibroblast.