Chromosomal DNA balance in human stem cell line 4BL.

TSitologiia i genetika Pub Date : 2016-07-01
H R Akopyan, V O Kushniruk, D O Mykytenko, N L Huleuk, Y Kremenskaya, L L Lukash
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Abstract

In the previous cytogenetic study of new human stem cell line 4BL at the 205th passage we observed the ploidy of chromosomal set and regular aberrations. To investigate the nature of monosomy of certain chromosomes the array CGH and FISH analyses have been used. The aberrations of chromosomes have been identified in all the cases of monosomies previously revealed by G-banding. The largest changes of the DNA balance have been detected in the chromosomes 2, 4, 10, 13 and 17. The probable cause of the monosomies of chromosomes 4, 10, 13 and 17 is massive loss of the genetic material. The monosomy of the second chromosome pair is caused by significant transformation one of the homologs in a type of numerous duplications and formation of der(2)t(2;?)(q21;?). Due to application of array CGH the regions of the structural aberrations of the chromosomes 2, 4, 10, 13 and 17 have been concretized, what permitted to perform their clarifying identification by multicolored FISH method. The results obtained by us confirm the hypothesis about coordinated appearance of the deletions and duplications and their stabilization impact on the transformed chromosomes.

人干细胞系4BL染色体DNA平衡。
在前人对新人类干细胞4BL第205代的细胞遗传学研究中,我们观察到染色体组的倍性和规律性畸变。为了研究某些染色体单体的性质,已经使用了阵列CGH和FISH分析。染色体畸变已被确定在所有的单体的情况下,以前揭示的g带。DNA平衡变化最大的是染色体2、4、10、13和17。4号、10号、13号和17号染色体单体的可能原因是遗传物质的大量丢失。第二对染色体的单体是由大量重复的同源物中的一个发生重大转变和der(2)t(2;?)(q21;?)的形成引起的。由于阵列CGH的应用,使染色体2、4、10、13和17的结构畸变区域具体化,从而允许用彩色FISH方法对其进行澄清鉴定。我们得到的结果证实了缺失和重复协调出现的假设,以及它们对转化染色体的稳定影响。
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